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  • Polymer and Materials Science  (7)
  • Glutamate synthase  (4)
  • 1
    Digitale Medien
    Digitale Medien
    Antigenic similarities between ferredoxin-dependent nitrite reductase and glutamate synthase from Chlamydomonas reinhardtii
    Amsterdam : Elsevier
    Biochimica et Biophysica Acta (BBA)/Protein Structure and Molecular 957 (1988), S. 152-157 
    Details
    ISSN: 0167-4838
    Schlagwort(e): (C. reinhardtii) ; Antigenic similarity ; Ferredoxin dependent enzyme ; Glutamate synthase ; Nitrite reductase
    Quelle: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Thema: Biologie , Chemie und Pharmazie , Medizin
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1016/0167-4838(88)90168-9
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  • 2
    Digitale Medien
    Digitale Medien
    Immunological approach to subunit composition of ferredoxin-nitrite reductase from Chlamydomonas reinhardtii
    Amsterdam : Elsevier
    Plant Science 95 (1993), S. 9-21 
    Details
    ISSN: 0168-9452
    Schlagwort(e): Chlamydomonas reinhardtii ; Glutamate synthase ; Immunoaffinity purification ; Immunological comparison ; Nitrite reductase ; Proteolysis
    Quelle: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Thema: Biologie
    Materialart: Digitale Medien
    URL: http://linkinghub.elsevier.com/retrieve/pii/0168-9452(93)90074-A
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  • 3
    Digitale Medien
    Digitale Medien
    Purification and molecular properties of ferredoxin-glutamate synthase from Chlamydomonas reinhardii (1984)
    Springer
    Planta 162 (1984), S. 180-187 
    Details
    ISSN: 1432-2048
    Schlagwort(e): Chlamydomonas (ferredoxin-glutamate synthase) ; Ferredoxin-glutamate synthase ; Glutamate synthase
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract Ferredoxin-glutamate synthase (EC 1.4.7.1) from Chlamydomonas reinhardii has been purified to electrophoretic homogeneity, with a specific activity of 10.4 units mg-1 protein, by a method which included chromatography on diethylaminoethyl sephacel and hydroxylapatite, and ferredoxin-sepharose affinity treatment. The enzyme is a single polypeptide chain of M r 146000 dalton which shows an absorption spectrum with maxima at 278, 377 and 437 nm, and an A276/A437 absorptivity ratio of 7.0. The anaerobic addition of dithionite results in the loss of the absorption peak at 437 nm, which is restored upon reoxidation of the enzyme with an excess of 2-oxoglutarate, alone or in the presence of glutamine. This indicates the presence in the enzyme of a flavin prosthetic group, which is functional during the catalysis. The ferredoxin-glutamate synthase can be assayed with methyl viologen, chemically reduced with dithionite, but it is unable to use reduced pyridine nucleotide. Azaserine, 6-diazo-5-oxo-norleucine, bromocresol green and p-hydroxymercuribenzoate are potent inhibitors of this activity, which, on the other hand, is stable upon heating at 45°C for 10 min.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00410216
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  • 4
    Digitale Medien
    Digitale Medien
    Cloning and sequence analysis of a cDNA for barley ferredoxin-dependent glutamate synthase and molecular analysis of photorespiratory mutants deficient in the enzyme (1993)
    Springer
    Planta 189 (1993), S. 475-483 
    Details
    ISSN: 1432-2048
    Schlagwort(e): Amidotransferase ; Amino-terminal sequences ; Chromosomal assignment ; Glutamate synthase ; Hordeum (mutants) ; Photorespiration mutants (barley) ; Polymerase chain reaction
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract The NH2-terminal sequences of ferredoxin-dependent glutamate synthase (Fd-GOGAT; EC 1.4.7.1) purified from barley (Hordeum vulgare L.) and Chlamydomonas reinhardtii (Dangeard), and of a barley peptide, were determined and the barley sequences were used to design oligonucleotide primers for the polymerase chain reaction. A specific 1.3-kilobase (kb) cDNA fragment specifying the NH2-terminal one-third of the mature barley polypeptide, was amplified, cloned and sequenced. The NH2-terminus of plant Fd-GOGAT is highly conserved and homologous to the NH2-terminus of the heavy subunit of Escherichia coli NADPH-GOGAT. Based on sequence homologies, we tentatively identified the NH2-terminal region of Fd-GOGAT as the glutamine-amidotransferase domain, which is related to the corresponding domain of the purF-type amidotransferases. The Fd-GOGAT cDNA clone, and polyclonal antibodies raised against the barley enzyme, were used to analyse four Fd-GOGAT-deficient photorespiratory mutants. Three mutants (RPr 82/1, RPr 82/9 and RPr 84/82) had no detectable Fd-GOGAT protein in leaves, while the fourth (RPr 84/42) had a small amount of cross-reacting material. Hybridization to Northern blots of total leaf RNA revealed that both RPr 82/9 and RPr 84/82 were indistinguishable from the parental line (Maris Mink), having normal amounts of a 5.7-kb mRNA species. On the other hand, RPr 82/2 and RPr 84/42 each contained two distinct hybridizing RNA species, one of which was larger than 5.7 kb, the other smaller. Using a set of wheat-barley telosomic addition lines we have assigned the Fd-GOGAT structural locus to the short arm of chromosome 2.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00198209
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  • 5
    Digitale Medien
    Digitale Medien
    Terpolymérisation acrylonitrile-styrène-acrylate de méthyle, 4Partie 3: cf. J. Guillot, L. Rios Guerrero, Makromol. Chem. 183, 1979 (1982). Copolymérisation en emulsion styrène-acrylate de méthyle en réacteur fermé (batch) en présence de persulfate de potassium (1988)
    New York : Wiley-Blackwell
    Die Makromolekulare Chemie 189 (1988), S. 361-377 
    Details
    ISSN: 0025-116X
    Schlagwort(e): Chemistry ; Polymer and Materials Science
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Chemie und Pharmazie , Physik
    Notizen: To improve the knowledge of emulsion copolymerization of monomers both swelling their copolymers, but which are of quite different polarity (water solubility), a series of styrene (S)/methyl acrylate (MeA) copolymerizations was carried out in batch at 50°C with potassium persulfate as initiator. The overall rates of copolymerization increase with the amount of MeA in the monomer feed. Copolymer composition follows the usual copolymerization equation if bulk/solution reactivity ratios (rij) and monomer partition between aqueous and organic phase are taken into account (simulation). However, accurate kinetic data at low conversion (gas chromatography) put in evidence an enhanced polymerization of the more hydrophilic monomer (MeA), which can be attributed to polymerization in the water phase. Particle sizes increase with conversion and tend to a limiting value, the higher the MeA content is. Particle number (Np), which is practically constant with conversion of S homopolymerization, tends to increase with MeA content as polymerization proceeds. This trend is enhanced if the emulsifier (sodium dodecanesulfonate, SDS) concentration is increased. Overall propagation rate constants were estimated as function of the experimental conditions and monomer concentration within the particles. From kinetic data (rate of polymerization) and Np, it was found that the average number of radicals per particle, ñ, remains close to 0,5. It was then possible considering S(kp = 125 1 · mol-1 · s-1) as a standard monomer, to estimate the polymerization rate constant for MeA (335 1 · mol-1 · s-1). Since adsorption of emulsifier was shown to be closely related to particle surface composition, the specific area As of SDS was measured on latices at various conversions and initial monomer feeds. As conversions increases, the particle surface appears to be richer and richer in MeA, which corresponds to a particle structuration. Strong and weak acid group titration is also in quite good agreement with the colloidal behaviour.
    Zusätzliches Material: 11 Ill.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1002/macp.1988.021890211
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  • 6
    Digitale Medien
    Digitale Medien
    Terpolymérisation acrylonitrile-styrène-acrylate de méthyle, 5Partie 4: cf.1. Copolymérisation en emulsion styrène-acrylate de méthyle en réacteur fermé en présence du styrène amorceur redox persulfate the potassium/hydrogénodisulfite de sodium (1988)
    New York : Wiley-Blackwell
    Die Makromolekulare Chemie 189 (1988), S. 379-397 
    Details
    ISSN: 0025-116X
    Schlagwort(e): Chemistry ; Polymer and Materials Science
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Chemie und Pharmazie , Physik
    Notizen: A series of emulsion copolymerizations of styrene (S) with methyl acrylate (MeA) initiated by the redox system potassium persulfate (KPS)/sodium hydrogen disulfite (SBS) was carried out at 50°C. The monomer feed composition and the monomer/water (M/W) ratios were widely varied. The polymerization rate was found to be higher than in the case when only KPS was applied. Initial (influence of polymerization in water phase) and final copolymer molecules (accumulation of MeA in the feed due to a lower reactivity) are richer in the more hydrophilic monomer (MeA). It could be shown that a high water solubility makes the emulsion reactivity ratios to be “apparent” kinetic parameters, which result in a higher number of azeotropic compositions, compared to bulk or solution, according to the M/W ratio. Particle sizes are smaller and the size distribution is larger than in polymerizations in the presence of KPS alone. Particle number (Np) tends to increase with conversion for monomer mixtures rich in MeA, contrary to S homopolymerizations in which Np remains constant. Comparison of Np and kinetic data suggests that only part of the particles in actually active in polymerization (N*p). N*p can be estimated assuming that the average number of radical per particle, ñ remains close to 0,5 - as found in a previous study - which gives also an estimate of very tiny particles generated at low conversion and undetectable by the usual QELS and MET techniques. The experimental data, to some extent, allow to quantify the competition between micellar and homogeneous nucleation mechanisms.
    Zusätzliches Material: 15 Ill.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1002/macp.1988.021890212
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  • 7
    Digitale Medien
    Digitale Medien
    The glass transition in linear low density polyethylene determined by thermally stimulated depolarization currents (1996)
    Bognor Regis [u.a.] : Wiley-Blackwell
    Journal of Polymer Science Part B: Polymer Physics 34 (1996), S. 641-648 
    Details
    ISSN: 0887-6266
    Schlagwort(e): polyethylene ; glass transition ; TSDC ; γ transition ; β transition ; Chemistry ; Polymer and Materials Science
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Chemie und Pharmazie , Physik
    Notizen: New thermally stimulated depolarization currents (TSDC) results on LLD polyethylene functionalized with diethylmaleate polar groups are precisely computer fitted with the direct signal analysis technique. It is shown that the TSDC spectrum consists, with increasing temperatures, of a sub-γ peak, a sharp γ peak, and a β and an α relaxation. The first peak is analyzed in terms of Arrhenius relaxation times, whereas the γ and β transitions could only be fitted by using Vogel-Fulcher temperature dependence for the relaxation times. The best value for To obtained from both fittings is 69.7 K. This is a quantitative proof for the identification of the γ transition as one of the dielectric manifestations of the glass-rubber transition for polyethylenes, Tg = 136.5 K, which has been discussed extensively in the literature. The β relaxation, Tgβ = 237 K, has also the expected characteristic of a glass transition; the existence of two Tgs in polyethylene could explain our results. © 1996 John Wiley & Sons, Inc.
    Zusätzliches Material: 6 Ill.
    Materialart: Digitale Medien
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  • 8
    Digitale Medien
    Digitale Medien
    Spectroscopic studies of surface copper spinels. Influence of pretreatments on chemical state of copper (1993)
    Chichester [u.a.] : Wiley-Blackwell
    Surface and Interface Analysis 20 (1993), S. 1067-1074 
    Details
    ISSN: 0142-2421
    Schlagwort(e): Chemistry ; Polymer and Materials Science
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Physik
    Notizen: The nature of copper species of a series of alumina-supported copper samples was investigated by the kinetics of reduction, x-ray photoelectron spectroscopy, temperature-programmed desorption-mass spectrometry of chemisorbed NO and infrared spectroscopy of chemisorbed NO and CO probes. The role of these copper species was also examined in the catalytic decomposition of NO. The surface properties of copper were found to depend strongly on both Cu loading and calcination treatment. At Cu contents as low as 0.6 wt.%, Cu2+ ions from a well-dispersed interacting phase at the alumina surface, which upon calcination forms a surface spinel CuAl2O4. At copper contents up to 3.2 wt.%, both the surface spinel CuAl2O4 and a segregated CuO phase were clearly distinguished. The partial reduction of these species upon thermal treatment under high vacuum, the ability of the surface sites to chemisorb NO and CO probes and their implications in the catalytic decomposition of NO are discussed.
    Zusätzliches Material: 6 Ill.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1002/sia.740201307
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  • 9
    Digitale Medien
    Digitale Medien
    SIMS matrix effects in Alx Ga1 - xAs: Influence of instrumental parameters (1989)
    Chichester [u.a.] : Wiley-Blackwell
    Surface and Interface Analysis 14 (1989), S. 31-38 
    Details
    ISSN: 0142-2421
    Schlagwort(e): Chemistry ; Polymer and Materials Science
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Physik
    Notizen: The influence of instrumental parameters on the matrix effects (ion and sputtering yield variations) observed in AlxGa1 - xAs are critically evaluated. Ion and sputtering yield data from three different SIMS instruments (ARL IMMA, Cameca IMS-3F and Atomika SIMS) are examined. The influence of primary ion (O2 +) energy and incident angle on the extent of surface oxidation was examined using AES. Although relative ion yields varied linearly with the matrix composition in all instances, these ion yield variations differed greatly (a factor of 50) between instruments. These differences were attributed to differences in the surface oxygen concentrations produced by the SIMS instruments. AlAs was shown to be completely oxidized under each of the SIMS ion bombardment conditions. However, the oxygen levels incorporated in GaAs and the other Al poor specimens depended largely on the incident angle and to a lesser extent on the ion energy. Small incidence angles (normal incidence) promoted the most extensive oxidation of the Al-poor specimens, resulting in the smallest ion yield variations with matrix. As with the relative ion yields, relative sputtering yields varied linearly with the matrix composition in all instances. However, in contrast to relative ion yields, the relative sputtering yields did not change significantly under the different instrumental conditions. It was concluded that incident angle and ion energy had approximately the same influence on the sputtering yields obtained from the reference and the sample matrices. Thus, the relative sputtering yields were insensitive to changes in these ion beam parameters.
    Zusätzliches Material: 4 Ill.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1002/sia.740140109
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  • 10
    Digitale Medien
    Digitale Medien
    Methyl methacrylate polymerization in the presence of polystyrene (1978)
    New York : Wiley-Blackwell
    Journal of Polymer Science: Polymer Chemistry Edition 16 (1978), S. 3003-3006 
    Details
    ISSN: 0360-6376
    Schlagwort(e): Physics ; Polymer and Materials Science
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Chemie und Pharmazie
    Zusätzliches Material: 1 Ill.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1002/pol.1978.170161127
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