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  • Automatic control  (1)
  • Glucose repression  (1)
  • 1990-1994  (2)
  • 1
    ISSN: 1432-0983
    Keywords: Saccharomyces cerevisiae ; Fructose-1,6-bisphosphatase ; Glucose repression ; Gene activation ; Gluconeogenesis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Fructose-1,6-bisphosphatase is a key enzyme in gluconeogenesis and the FBP1 gene is not transcribed during growth with glucose. Genetic analysis indicated a positive regulation of FBP1 expression after exhaustion of glucose. By linker-deletion analysis, two upstream activation sites (UAS1 and UAS2) were localized and the respective UAS-binding factors (DAP I and DAP II for derepression activating protein) were identified by gel retardation. UAS1 and UAS2 span about 30 bp each, and are separated by approximately 30 bp. Both UAS sites act synergistically. Although UAS1 showed some similarities to the DNA-binding consensus for the general yeast activator Rap1, competition experiments and DEAE-chromatography proved that DAP I and Rap1 correspond to different proteins. Gel retardation by DAP I depended on carbon sources and did not occur in cells growing logarithmically with glucose, whereas a strong retardation signal was obtained with ethanol-grown cells. The present results suggest that DAP I and DAP II are the final regulatory elements for glucose derepression.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Nutrient cycling in agroecosystems 33 (1992), S. 1-34 
    ISSN: 1573-0867
    Keywords: Automatic control ; fertilizer application ; geographic information systems ; sensors ; sitespecific ; spatially-variable ; variability
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The variability of crops, soils, and pests within crop production fields has led to attempts to understand those variations and to manage crop production accordingly. The rapidly expanding research and development on the management and control of crop production according to in-field variations is reviewed. The various stages and components of this spatially-variable control are classified and presented along with a proposed terminology and notation. Advances in component technologies (sensors, actuators, locators, geostatistics, geographic information systems) and their integration should reduce crop production costs and be environmentally advantageous.
    Type of Medium: Electronic Resource
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