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  • Glucagon  (2)
  • ATP  (1)
  • 1
    Digitale Medien
    Digitale Medien
    Amsterdam : Elsevier
    Peptides 10 (1989), S. 1195-1197 
    ISSN: 0196-9781
    Schlagwort(e): Amino acid sequence ; Glucagon ; Insulin ; New World marsupial ; Opossum ; Pancreatic polypeptide
    Quelle: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Thema: Chemie und Pharmazie
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
    BibTip Andere fanden auch interessant ...
  • 2
    Digitale Medien
    Digitale Medien
    Amsterdam : Elsevier
    Peptides 11 (1990), S. 683-685 
    ISSN: 0196-9781
    Schlagwort(e): Amino acid sequence ; Chemical assay ; Chinchilla ; Glucagon ; Pancreatic polypeptide ; Purification
    Quelle: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Thema: Chemie und Pharmazie
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
    BibTip Andere fanden auch interessant ...
  • 3
    ISSN: 1432-1424
    Schlagwort(e): KBIC ; Inward rectifier ; Cystic fibrosis ; ATP ; cAMP ; Protein kinase
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Chemie und Pharmazie
    Notizen: Abstract Agonists that elevate calcium in T84 cells stimulate chloride secretion by activating KBIC, an inwardly rectifying K channel in the basolateral membrane. We have studied the regulation of this channel by calcium, nucleotides and phosphorylation using patch clamp and short-circuit current (I SC) techniques. Open probability (P 0) was independent of voltage but declined spontaneously with time after excision. Rundown was slower if patches were excised into a bath solution containing ATP (10 μm−5 mm), ATP (0.1 mm) + protein kinase A (PKA; 180 nm), or isobutylmethylxanthine (IBMX; 1 mm). Analysis of event durations suggested that the channel has at least two open and two closed states, and that rundown under control conditions is mainly due to prolongation of the long closed time. Channel activity was restimulated after rundown by exposure to ATP, the poorly hydrolyzable ATP analogue AMP-PNP, or ADP. Activity was further enhanced when PKA was added in the presence of MgATP, but only if free calcium concentration was elevated (400 nm). Nucleotide stimulation and inward rectification were both observed in nominally Mg-free solutions. cAMP modulation of basolateral potassium conductance in situ was confirmed by measuring currents generated by a transepithelial K gradient after permeabilization of the apical membrane using α-toxin. Finally, protein kinase C (PKC) inhibited single KBIC channels when it was added directly to excised patches. These results suggest that nonhydrolytic binding of nucleotides and phosphorylation by PKA and PKC modulate the responsiveness of the inwardly rectifying K channel to Ca-mediated secretagogues.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
    BibTip Andere fanden auch interessant ...
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