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  • 1
    Electronic Resource
    Electronic Resource
    Ribosomal DNAs: an exception to the conservation of gene order in rice genomes (2000)
    Springer
    Molecular genetics and genomics 263 (2000), S. 586-591 
    Details
    ISSN: 1617-4623
    Keywords: Key words rDNA locus ; Fluorescence in situ hybridization (FISH) ; Genus Oryza ; Comparative genomics
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract rDNA (18S-5.8S-25S rDNA) and 5S rDNA loci were visualized on the chromosomes of six species of the genus Oryza by fluorescence in situ hybridization (FISH) and the labeled rice chromosomes were identified based on their condensation patterns. As a result, the chromosomes harboring rDNA and/or 5S rDNA loci were determined in the complement for all the known rice genomes. Variation in the location of the rDNA loci indicated the transpositional nature of the rDNAs in the genus Oryza, as also suggested in Triticeae and Allium. Comparative analysis of the locations of rDNA loci among rice, maize and wheat revealed that variability in the physical location of the rDNA loci was characteristic of the genus Oryza and also of the genera of Gramineae. This variability in the location of the rDNA loci between evolutionarily related species is in sharp contrast to the conservation of the general order of genes in their genomes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004380051205
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  • 2
    Electronic Resource
    Electronic Resource
    Variability in rDNA loci in the genus Oryza detected through fluorescence in situ hybridization (1994)
    Springer
    Theoretical and applied genetics 87 (1994), S. 893-899 
    Details
    ISSN: 1432-2242
    Keywords: Fluorescence in-situ hybridization (FISH) ; Ribosomal DNA ; Genus Oryza ; Image analysis ; NOR variability
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The 17s-5.8s-25s ribosomal RNA gene (rDNA) loci in Oryza spp. were identified by the fluorescence in-situ hybridization (FISH) method. The rDNA loci were located on one-to-three chromosomes (two-to-six sites) within the eight diploid Oryza spp. One of the rDNA loci gave the weakest hybridization signal. This locus is reported for the first time in the genus Oryza. The chromosomes containing the rDNA loci were determined to be numbers 9, 10 and 11 in descending order of the copy number of rDNA. The application of image analysis methods, after slide preparation treatments (post-treatments), and the use of a thermal cycler, greatly improved the reproducibility of the results. The evolutionary significance of the variability of rDNA loci among the Oryza spp. is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00225782
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  • 3
    Electronic Resource
    Electronic Resource
    Identification of the rice D-genome chromosomes by genomic in situ hybridisation (1997)
    Springer
    Theoretical and applied genetics 95 (1997), S. 1239-1245 
    Details
    ISSN: 1432-2242
    Keywords: Key words Genomic in situ hybridisation (GISH) ; Genus Oryza ; O. latifolia (CCDD) ; O. minuta (BBCC) ; Rice D genome
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  The 24 rice D-genome chromosomes were identified among the 48 chromosomes of O. latifolia, which comprise the C- and D-genomes, using genomic in situ hybridisation (GISH). The B-genome chromosomes were also discriminated from the C-genome chromosomes in O. minuta (BBCC) by GISH. A comparison of the differences in the fluorescence intensity between the C and D genomes within O. latifolia (CCDD), and between the B and C genomes within O. minuta, indicated that the overall nucleotide-sequence homology between the B and C genomes is less than that between the C and D genomes. The origin of the D genome and the phylogenetic relationship of the D genome among the rice genomes are discussed, based on the results obtained.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001220050687
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