ISSN:
1617-4623
Keywords:
Key words Gangrene
;
Toxins
;
Enterotoxin
;
Plasmids
;
Genome mapping
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract The intron-encoded endonuclease I-CeuI from Chlamydomonas eugametos was shown to cleave the circular chromosomes of all Clostridium perfringens strains examined at single sites in the rRNA operons, thereby generating ten fragments suitable for the rapid mapping of virulence genes by pulsed-field gel electrophoresis (PFGE). This method easily distinguishes between plasmid and chromosomal localisations, as I-CeuI only cuts chromosomal DNA. Using this approach, the genes for three of the four typing toxins, β, ɛ, and ι, in addition to the enterotoxin and λ-toxin genes, were shown to be plasmid-borne. In a minority of strains, associated with food poisoning, where the enterotoxin toxin gene was located on the chromosome, genes for two of the minor toxins, θ and μ, were missing.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF02174122
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