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  • 1
    Electronic Resource
    Electronic Resource
    Characterization of chloroplast DNA in Chlamydomonas eugametos and C. moewusii and its inheritance in hybrid progeny (1980)
    Springer
    Current genetics 2 (1980), S. 139-147 
    Details
    ISSN: 1432-0983
    Keywords: Chlamydomonas ; Chloroplast ; DNA ; Genetics
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The density, molecular weight, and cellular repetition of DNA molecules associated with the β-DNA satellite of the interfertile algae Chlamydomonas eugametos and C. moewusii are reported. The similarities between these values and those for the chloroplast DNA (cpDNA) in the related alga Chlamydomonas reinhardtii indicate that these satellites represent cpDNA. The buoyant densities of C. eugametos and C. moewusii cpDNAs are indistinguishable from one another, as are those of their respective nuclear DNAs. These densities differ slightly from the densities of the homologous components of C. reinhardtii whole cell DNA. All three species differ with respect to additional minor satellite DNAs and low molecular weight DNAs of unknown cellular location. Differences in the Aval and Smal restriction endonuclease fragmentation patterns of C. eugametos and C. moewusii cpDNAs were employed to study the inheritance of cpDNA in an F1 hybrid which had inherited a non-Mendelian streptomycin resistance marker (sr-2) from the C. eugametos mating-type plus (mt +) parent and in two homoplasmic mitotic segregants from a B 1 hybrid (F1 × C. moewusii) which had been initially heteroplasmic for the resistance marker. Although the cpDNA patterns in the F1 hybrid were similar to those of the C. eugametos ml 1 parent, important differences were noted which suggest that recombination between C. eugametos and C. moewusii cpDNA had occurred. Homoplasmic streptomycin resistant and sensitive mitotic segregants recovered from the B1 hybrid product reveal Aval restriction patterns similar to those of the respective resistant and sensitive parents. These data are consistent with the hypothesis that the sr-2 marker is located in cpDNA and that C. eugametos and C. moewusii cpDNA sequences can coexist in the same chloroplast and, at least sometimes, segregate without extensive recombination. The transmission of low molecular weight DNAs characteristic of C. moewusii but of unknown cellular origin shows no direct correlation with the transmission of the sr-2 marker.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00420626
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  • 2
    Electronic Resource
    Electronic Resource
    Histone genes in higher plants: Organization and expression (1987)
    Chichester [u.a.] : Wiley-Blackwell
    Developmental Genetics 8 (1987), S. 461-473 
    Details
    ISSN: 0192-253X
    Keywords: H3 and H4 genes ; plants ; structure ; cloning ; S1 mapping ; Life and Medical Sciences ; Genetics
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: The general structure of the plant histone genes has been deduced from the comparison of the nucleotide sequences of ten H3 and H4 genes of maize (3 H3 and 3 H4) and Arabidopsis thaliana (2 H3 and 2 H4). The five H3 and five H4 genes encode the same proteins, respectively. The 5′-flanking regions contain the classical histone gene-specific consensus sequences. In addition, a conserved octanucleotide CGCGGATC was found in all plant histone genes at 200-250 nucleotides before the initiation codon.All six maize H3 and H4 genes are transcribed during early germination as shown by nuclease S1 mapping and reverse transcriptase primer extension experiments. The mRNA 5′-ends are located within the consensus sequence CCAA/CT/C. The 3′-ends lack the classical T-hyphenated dGC-rich palindromic structure and possess long nontranslated sequences.In both plants the multiple copies of the H3 and H4 genes are organized into multigenic families. The genes of each family show a similar proximal environment, suggesting that they originate from the duplication of a common ancestor.
    Additional Material: 7 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/dvg.1020080512
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  • 3
    Electronic Resource
    Electronic Resource
    Regulatory elements and interactions in the Drosophila 68C glue gene cluster (1989)
    Chichester [u.a.] : Wiley-Blackwell
    Developmental Genetics 10 (1989), S. 189-197 
    Details
    ISSN: 0192-253X
    Keywords: Drosophila ; transformation ; Glue gene ; Gene cluster ; Gene regulation ; Sgs-3 ; Life and Medical Sciences ; Genetics
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: We reviewed studies on the developmental regulation of the 68C glue gene cluster of Drosophila melanogaster. Extensive transformation analyses of Sgs-3 have shown that four regions necessary for normal expression can be distinguished. The first ( + 10 to -50) contains the transcription start site and TATA motif. This region can be replaced functionally by corresponding sequences from the hsp70 gene, but it is sensitive to point mutations in the TATA sequence. The second region (-50 to -98) contains more than one upstream sequence that, in combination with the other elements, leads to stage and tissue-specific expression. The third region (centered at -600) contains an element that enhances transcript levels some 20-fold. The final region (between -1.65 and -2.35 kb) contains elements having modest (twofold to threefold) effects on expression, one of which is contained in the coding sequences of Sgs-7, a second member of the cluster.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/dvg.1020100308
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  • 4
    Electronic Resource
    Electronic Resource
    A haploid and a diploid cell cycle coexist in an in vitro immortalized spermatogenic cell line (1995)
    Chichester [u.a.] : Wiley-Blackwell
    Developmental Genetics 16 (1995), S. 119-127 
    Details
    ISSN: 0192-253X
    Keywords: Germ cell line ; FACS ; mitosis ; meiosis ; Life and Medical Sciences ; Genetics
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: We have recently established a conditionally immortalized germ cell line [GC-2spd(ts)] that, at the permissive temperatures of 37°C and 32°C, is able to undergo meiosis in vitro and form round spermatids [Hofmann et al., (1994): Proc Natl Acad Sci USA 91:5533-5537]. In this report, we provide data that indicate that the GC-2spd(ts) cell line consists of two cell populations undergoing a haploid (n/2n) cell cycle and a diploid (2n/4n) cell cycle, respectively. The cells containing 2n DNA, when sorted by fluorescence-activated cell sorting, are able to reconstitute the full population of n/2n/4n DNA cells, indicating that they are able to commit to the reductive meiotic division and form haploid spermatids or to continue self-replication through a diploid cell cycle. This GC-2spd(ts) cell line provides a valuable tool to study the molecular mechanisms involved in the cellular decision between self-renewal by mitosis and commitment to meiosis. © 1995 Wiley-Liss, Inc.
    Additional Material: 8 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/dvg.1020160205
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  • 5
    Electronic Resource
    Electronic Resource
    X. Yeast sequencing reports. Revised nucleotide sequence of the cor region of yeast Saccharomyces cerevisiae chromosome X (1994)
    New York, NY [u.a.] : Wiley-Blackwell
    Yeast 10 (1994), S. 811-818 
    Details
    ISSN: 0749-503X
    Keywords: Yeast ; Saccharomyces cerevisiae ; chromosome X ; COR cluster ; genes CYC1 ; UTR1 ; UTR3 ; OSM1 ; tRNAGly ; RAD7 ; open reading frame: systematic sequencing ; Life and Medical Sciences ; Genetics
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: The COR region, a gene cluster located on chromosome X of Saccharomyces cerevisiae and including genes CYC1, UTR1, UTR3, OSM1, tRNAGly and RAD7, was sequenced within the framework of the European Union genome systematic sequencing project. It was compared with previously published sequences to be found in GenBank under the acronym YSCCORA. While some of the discrepancies observed can be readily ascribed to polymorphism, others most probably result from sequencing errors. A revised version of the sequence of the COR cluster is given. The sequence has been deposited in the EMBL Data Library under Accession Number L26347.
    Additional Material: 1 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/yea.320100611
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  • 6
    Electronic Resource
    Electronic Resource
    A family of laboratory strains of Saccharomyces cerevisiae carry rearrangements involving chromosomes I and III (1998)
    New York, NY [u.a.] : Wiley-Blackwell
    Yeast 14 (1998), S. 551-564 
    Details
    ISSN: 0749-503X
    Keywords: yeast ; S. cerevisiae ; genetics ; karyotypes ; chromosomal rearrangements ; Life and Medical Sciences ; Genetics
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: In order to study meiotic segregation of chromosome length polymorphism in yeast, we analysed the progeny of a cross involving two laboratory strains FL100trp and YNN295. Analysis of the parental strains led us to detect an important length polymorphism of chromosomes I and III in FL100trp. A reciprocal translocation involving 80 kb of the left arm of chromosome III and 45 kb of the right arm of chromosome I was shown to be the cause for the observed polymorphism in this strain. The characterization of the translocation breakpoints revealed the existence of a transposition hot-spot on chromosome I: the sequence of the translocation joints on chromosomes I and III suggests that the mechanism very likely involved homologous recombination between Ty2 transposable elements on each chromosome. Analysis of FL100, FL200 and FL100trp ura, which are related to FL100trp, shows that this reciprocal translocation is present in some of the strains of the FL series, whereas the parental strain FL100 does not carry the same rearrangement. We evidenced instead the duplication of 80 kb of chromosome III on chromosome I and a deletion of 45 kb of the right arm of chromosome I in this strain, indicating that secondary events might have taken place and that the strain currently named FL100 is not the common ancestor of the FL series. © 1998 John Wiley & Sons, Ltd.
    Additional Material: 7 Ill.
    Type of Medium: Electronic Resource
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  • 7
    Electronic Resource
    Electronic Resource
    Analysis of a 42·5 kb DNA sequence of chromosome X reveals three tRNA genes and 14 new open reading frames including a gene most probably belonging to the family of ubiquitin-protein ligases (1995)
    New York, NY [u.a.] : Wiley-Blackwell
    Yeast 11 (1995), S. 775-781 
    Details
    ISSN: 0749-503X
    Keywords: Saccharomyces cerevisiae ; chromosome X ; open reading frames ; tRNA ; ubiquitin-dependent proteolytic pathway ; Life and Medical Sciences ; Genetics
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: We have sequenced a 42,500 bp stretch located on chromosome X of Saccharomyces cerevisiae between the genes MET3 and CDC8. This stretch contains 24 open reading frames (ORFs) of at least 100 amino acids. Ten of these correspond to previously published sequences, whereas of the 14 remaining ORFs, only one, GTD892, has significant similarity to proteins from yeast or other organisms. It may belong to the family of ubiquitin-protein ligases and be involved in the ubiquitin-dependent proteolytic pathway. In addition, three tRNA genes were recognized, two of which had not been hitherto localized. The sequence has been deposited in the Genome Sequence Data Base under Accession Number L36344.
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/yea.320110809
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  • 8
    Electronic Resource
    Electronic Resource
    Rapid identification and characterization of peroxisomal assembly mutants in Yarrowia lipolytica (1993)
    New York, NY [u.a.] : Wiley-Blackwell
    Yeast 9 (1993), S. 507-517 
    Details
    ISSN: 0749-503X
    Keywords: Peroxisome ; immunofluorescence ; PTS-1 ; electroporation ; yeast ; targeting ; biogenesis ; Life and Medical Sciences ; Genetics
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: We describe the isolation and characterization of peroxisomal assembly mutants in the genetically manipulable yeast Yarrowia lipolytica (pay mutants). These mutants were initially identified as oleic acid-non-utilizers by their inability to grow on oleic acid, the utilization of which requires peroxisomal β-oxidation enzymes. Identification of a subset of oleic acid-non-utilizers as pay mutants was obtained by a rapid immunofluorescence procedure using antibodies to the peroxisomal targeting signal Ser-Lys-Leu-CO2H. Punctate structures characteristic of peroxisomes were not detected in pay mutants using this technique. This rapid identification by immunofluorescence should be generally applicable to the selection of peroxisomal assembly mutants in other yeasts. To take advantage of the pay mutant system, we constructed a genomic library in the autonomously replicating vector pINA445 and developed an efficient and rapid electroporation procedure for the functional complementation of these mutants. We have been successful in functionally complementing two independent pay mutants. Molecular analysis of these and other complementing genes will allow for characterization of some of the cellular elements involved in peroxisomal assembly.
    Additional Material: 6 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/yea.320090506
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  • 9
    Electronic Resource
    Electronic Resource
    The MAT locus revisited within a 9·8 kb fragment of chromosome III containing BUD5 and two new open reading frames (1991)
    New York, NY [u.a.] : Wiley-Blackwell
    Yeast 7 (1991), S. 881-888 
    Details
    ISSN: 0749-503X
    Keywords: Chromosome III ; Saccharomyces cerevisiae ; mating type ; HML ; BUD5 ; Life and Medical Sciences ; Genetics
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: This paper reports the DNA sequence of a segment of 9·8 kb of the chromosome III. The sequenced DNA contains the MATα locus. The new sequence of the MATα locus differs from the previously reported sequence by six modifications in the W segment. We have found the same modifications in the HML locus. The corrected sequence contains, in HML, an open reading frame (ORF) of 190 codons which ends at the border between the W segment and the flanking DNA. In the MAT locus, this ORF extends in the flanking DNA up to 538 codons. This ORF corresponds to a gene independently identified as BUD5 (Chant et al., 1991). This gene presents homologies with the exchange factors SDC25 and CDC25. A large ORF of 1399 codons is found on the opposite side of MATα (toward the telomere). This ORF corresponds to a new gene YCR724. Next to this gene is a small ORF, YCR725, of 127 codons. The localization of this fragment on chromosome III, originally supposed to be distal from the MAT locus based on genetic distance, illustrates variation in recombination frequency along the chromosome and suggests the existence of hot spots of recombination between MAT and the THR4 locus.
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/yea.320070815
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  • 10
    Electronic Resource
    Electronic Resource
    Stationary-Phase Gene Expression in Saccharomyces cerevisiae During Wine Fermentation (1997)
    New York, NY [u.a.] : Wiley-Blackwell
    Yeast 13 (1997), S. 903-915 
    Details
    ISSN: 0749-503X
    Keywords: wine yeasts ; alcoholic fermentation ; stationary phase ; nitrogen limitation ; Northern analysis ; heat-shock promoter ; HSP30::lacZ fusion ; Life and Medical Sciences ; Genetics
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: Genetic engineering of wine yeast strains requires the identification of gene promoters specifically activated under wine processing conditions. In this study, transcriptional activation of specific genes was followed during the time course of wine fermentation by quantifying mRNA levels in a haploid wine strain of Saccharomyces cerevisiae grown on synthetic or natural winery musts. Northern analyses were performed using radioactive probes from 19 genes previously described as being expressed under laboratory growth conditions or on molasses in S. cerevisiae during the stationary phase and/or under nitrogen starvation. Nine genes, including members of the HSP family, showed a transition-phase induction profile. For three of them, mRNA transcripts could be detected until the end of the fermentation. Expression of one of these genes, HSP30, was further studied using a HSP30::lacZ fusion on both multicopy and monocopy expression vectors. The production of β-galactosidase by recombinant cells was measured during cell growth and fermentation on synthetic and natural winery musts. We showed that the HSP30 promoter can induce high gene expression during late stationary phase and remains active until the end of the wine fermentation process. Similar expression profiles were obtained on five natural winery musts. © 1997 John Wiley & Sons, Ltd.
    Additional Material: 6 Ill.
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