ALBERT

All Library Books, journals and Electronic Records Telegrafenberg

X
feed icon rss

Your email was sent successfully. Check your inbox.

An error occurred while sending the email. Please try again.

Proceed reservation?

Export
  • 1
    Electronic Resource
    Electronic Resource
    Identification of polymorphic, conserved simple sequence repeats (SSRs) in cultivated Brassica species (1996)
    Springer
    Theoretical and applied genetics 93 (1996), S. 534-538 
    Details
    ISSN: 1432-2242
    Keywords: Key words DNA marker ; Genetic analysis ; Genetic diversity ; Genotyping ; Microsatellite
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The application of simple sequence repeat (SSR) genotyping for the characterization of genetic variation in crop plants has been hindered by ready access to useful primer pairs and potentially limited conservation of the repeat sequences among related species. In this phase of work, we report on the identification and characterization of SSRs that are conserved in Brassica napus L. (rapeseed) and its putative progenitors, B. oleracea L. (cabbage, and related vegetable types) and B. rapa (vegetable and oil types). Approximately 140 clones from a size-fractionated genomic library of B. napus were sequenced, and primer pairs were designed for 21 dinucleotide SSRs. Seventeen primer pairs amplified products in the three species and, among these, 13 detected variation between and within species. Unlike findings on SSR information content in human, no relationship could be established between the number of tandem repeats within the target sequence and heterozygosity. All primer pairs have been designed to work under identical amplification conditions; therefore, single-reaction, multiplex polymerase chain reaction (PCR) with these SSRs is possible. Once moderate numbers of primer pairs are accessible to the user community, SSR genotyping may provide a useful method for the characterization, conservation, and utilization of agricultural crop diversity.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001220050311
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 2
    Electronic Resource
    Electronic Resource
    Identification of polymorphic, conserved simple sequence repeats (SSRs) in cultivated Brassica species (1996)
    Springer
    Theoretical and applied genetics 93 (1996), S. 534-538 
    Details
    ISSN: 1432-2242
    Keywords: DNA marker ; Genetic analysis ; Genetic diversity ; Genotyping ; Microsatellite
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The application of simple sequence repeat (SSR) genotyping for the characterization of genetic variation in crop plants has been hindered by ready access to useful primer pairs and potentially limited conservation of the repeat sequences among related species. In this phase of work, we report on the identification and characterization of SSRs that are conserved in Brassica napus L. (rapeseed) and its putative progenitors, B. oleracea L. (cabbage, and related vegetable types) and B. rapa (vegetable and oil types). Approximately 140 clones from a size-fractionated genomic library of B. napus were sequenced, and primer pairs were designed for 21 dinucleotide SSRs. Seventeen primer pairs amplified products in the three species and, among these, 13 detected variation between and within species. Unlike findings on SSR information content in human, no relationship could be established between the number of tandem repeats within the target sequence and heterozygosity. All primer pairs have been designed to work under identical amplification conditions; therefore, single-reaction, multiplex polymerase chain reaction (PCR) with these SSRs is possible. Once moderate numbers of primer pairs are accessible to the user community, SSR genotyping may provide a useful method for the characterization, conservation, and utilization of agricultural crop diversity.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00417944
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 3
    Electronic Resource
    Electronic Resource
    Assessment of Genetic Diversity in Three Subsets Constituted from the ICRISAT Sorghum Collection Using Random vs Non-Random Sampling Procedures B. Using molecular markers (2000)
    Springer
    Theoretical and applied genetics 101 (2000), S. 197-202 
    Details
    ISSN: 1432-2242
    Keywords: Key words Core collection ; Sorghum ; SSRs ; Genetic diversity
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  The large size of the sorghum [Sorghum bi-color (L.) Moench] landrace collection maintained by ICRISAT lead to the establishment of a core collection. Thus, three subsets of around 200 accessions were established from: (1) a random sampling after stratification of the entire landrace collection (L), (2) a selective sampling based on quantitative characters (PCS), and (3) a selection based on the geographical origin of landraces and the traits under farmers’ selection (T). An assessment was done of the genetic diversity retained by each sampling strategy using the polymorphisms at 15 microsatellite loci. The landraces of each subset were genotyped with three multiplex polymerase chain reactions (PCRs) of five fluorescent primer-pairs each with semi-automated allele sizing. The average allelic richness for each subset was equivalent (16.1, 16.3 and 15.4 alleles per locus for the subsets PCS, L, and T, respectively). The average genetic diversity was also comparable for the three subsets (0.81, 0.77 and 0.80 for the subsets PCS, L, and T, respectively). Allelic frequency distribution for each subset was compared with a chi-square test but few significant differences were observed. A high percentage of rare alleles (71 to 76% of 206 total rare alleles) was maintained in the three subsets. The global molecular diversity retained in each subset was not affected by a sampling procedure based upon phenotypic characters.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001220051469
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
Close ⊗
This website uses cookies and the analysis tool Matomo. More information can be found here...