ISSN:
1617-4623
Keywords:
Glutamine synthetase
;
Arabidopsis thaliana
;
Gene expression
;
Light-regulation
;
Gene family
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Summary Glutamine synthetase (GS) plays an important role in the assimilation of nitrogen by higher plants. We present here a molecular analysis of the GS polypeptides, mRNAs, and genes of Arabidopsis thaliana. Western blot analysis of leaf and root protein extracts revealed at least two distinct GS polypeptides; 43 kDa and 39 kDa GS polypeptides were present in leaves, while only a 39 kDa GS was detected in roots. The 43 kDa GS polypeptide is light-inducible. In etiolated seedlings only the 39 kDa GS was detected. However, upon greening the 43 kDa GS increased to levels comparable to those observed in light-grown plants. Four distinct GS cDNA clones, λAtgsl1, λAtgsrl, λAtgsr2 and λAtk6 were isolated and characterized. Their complete nucleotide and deduced amino acid sequences are presented. The coding sequences of the four clones are 70–88% similar while their 5′ and 3′ untranslated regions exhibit less than 50% similarity. Northern blots of leaf, root and germinated seed RNA revealed that the four cDNAs hybridize to mRNAs which are differentially expressed in the organs of Arabidopsis thaliana. λAtgsl1 is leaf-specific and hybridizes to a 1.6 kb mRNA. Both λAtgsr1 and λAtgskb6 hybridize to 1.4 kb mRNAs which are expressed in both roots and germinated seeds. λAtgsr2 hybridizes to a 1.4 kb mRNA, which is primarily expressed in roots with low levels of expression in seeds and leaves. λAtgsl1, which represents the leaf-specific mRNA, is induced by light. λAtgsl1 mRNA levels increase during the greening of etiolated seedlings while λAtgsr1 levels remain constant. Southern blot analysis indicated that the Arabidopsis genome contains at least four and possibly five distinct GS genes.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00290662
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