ISSN:
1432-0878
Keywords:
Electron immunocytochemistry
;
Gastro-entero-pancreatic endocrine system
;
Gut
;
Pancreas
;
Prosomatostatin
;
Rat somatostatin cryptic peptide (RSCP)
;
Rat somatostatin-28
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Medicine
Notes:
Summary Antisera raised against rat somatostatin cryptic peptide (RSCP; corresponding to amino acids 63–77 of rat pro-somatostatin), somatostatin-28-(1–12) and somatostatin-28-(17–28) were used to compare the morphological distribution of these pro-somatostatin-derived sequences within the gastroenteropancreatic system of six mammalian species, including man. Using the immunogold staining procedure, RSCP, SS28-(1–12) and SS28-(17–28) immunoreactivity was found to be present in all the D cells of the tissues investigated. Extra-islet RSCP and SS28-(1–12) immunoreactive cells were also identified in some species. RSCP, SS28-(1–12) and SS28-(17–28) immunoreactivities were also present in a single case of human duodenal somatostatinoma. Immunostaining of serial ultrathin sections from all specimens in this study revealed that RSCP and both somatostatin immunoreactivities were co-localised in a majority of the reactive cells. Corroborative evidence was obtained by double immunogold staining which further showed that RSCP, SS28-(1–12) and SS28-(17–28) immunoreactivities were co-localised to individual secretory granules in D type cells, both normal and tumour. RSCP and SS28-(17–28) immunoreactivities were invariably co-localised, whereas SS28-(1–12) immunoreactivity was restricted to a sub-population of secretory granules. Our findings suggest that RSCP immunoreactivity is conserved in a number of mammalian species and is stored in each secretory granule type. Consequently, detection of the RSCP sequence may serve as a useful marker for somatostatin-producing systems throughout the diffuse neuroendocrine system.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00219018
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