ISSN:
0935-6304
Keywords:
Gas chromatography
;
Capillary columns
;
Discrimination factor non-vaporizing on-column/vaporizing splitless injection for underivatized steroids
;
Quantitation feasible for most underivatized steroid hormones except corticosteroids
;
Chemistry
;
Analytical Chemistry and Spectroscopy
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Chemistry and Pharmacology
Notes:
Recent developments in the preparation of capillary columns by persilanization of the glass surfaces have resulted in highly inactive, heat-stable columns. With this type of column certain steroid hormone classes can be analyzed without derivatization; these include the 17-keto steroids, 11-oxo compounds, estrogens, and progesterone metabolites. It has been found that good quantitative analysis calls for non-vaporizing, on-column injection, as normal vaporizing injection results in breakdown of thermolabile compounds such as estriol and 17-OH progesterone metabolites. The gas chromatographic method described was used to obtain urinary steroid profiles.
Additional Material:
12 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/jhrc.1240050402
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