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  • Gametophytic gene expression  (3)
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  • 1
    Electronic Resource
    Electronic Resource
    Gametophytic expression of genes controlling endosperm development in maize (1988)
    Springer
    Theoretical and applied genetics 75 (1988), S. 252-258 
    Details
    ISSN: 1432-2242
    Keywords: Gametophytic gene expression ; Defective endosperm mutants ; Pollen-style interaction
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary An indirect approach was adopted to select viable mutants affecting the male gametophytic generation in maize. This approach consists of a selection of endosperm defective mutants followed by a test for gametophytic gene expression, based on the distortion from mendelian segregation and on the measurement of pollen size and pollen sterility. The material used consisted of 34 endosperm defective viable mutants introgressed in B37 genetic background. Complementation tests indicated that the mutation in the collection of mutants affected different genes controlling endosperm development. The study of the segregation in F2 revealed four classes of de (defective endosperm) mutants: (1) mutants in which the mutation does not affect either gametophytic development or function; (2) mutants in which the effect on the gametophyte affects pollen development processes; (3) mutants showing effects on both pollen development and function, and (4) mutants where only pollen tube growth rate is affected. Positive and negative interactions between pollen and style were detected by means of mixed pollination (pollen produced by de/de plants and pollen from an inbred line used as a standard and carrying genes for colored aleurone), on de/de and de/ + plants. Positive interactions were interpreted as methabolic complementation between defective pollen and normal styles.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00303961
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  • 2
    Electronic Resource
    Electronic Resource
    gaMS-1: a gametophytic male sterile mutant in maize (1996)
    Springer
    Sexual plant reproduction 9 (1996), S. 216-220 
    Details
    ISSN: 1432-2145
    Keywords: Key words Maize pollen ; Male sterility ; Microsporogenesis ; Gametophytic gene expression
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  Several pollen-specific genes from different species have been isolated and characterized at the molecular level, but the precise role of most of them is unknown. Mutant analysis represents a direct approach to uncovering gene function, but the paucity of available mutants affecting pollen development and/or function and the poor characterization of the known mutants have so far limited the exploitation of this approach. Here we present the cytological characterization of gametophytic male sterile-1 (gaMS-1), a maize mutant that we identified in a program of transposon insertion mutagenesis for the production of mutations in gametophytically acting genes involved in microsporogenesis. gaMS-1 is expressed during or immediately after the first microspore division and leads to the production of immature, non-functional pollen grains. The mutation appears to affect the events leading to the developmental switch that follows the first microspore mitosis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004970050034
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  • 3
    Electronic Resource
    Electronic Resource
    gaMS-1: A gametophytic male sterile mutant in maize (1996)
    Springer
    Sexual plant reproduction 9 (1996), S. 216-220 
    Details
    ISSN: 1432-2145
    Keywords: Maize pollen ; Male sterility ; Microsporogenesis ; Gametophytic gene expression
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Several pollen-specific genes from different species have been isolated and characterized at the molecular level, but the precise role of most of them is unknown. Mutant analysis represents a direct approach to uncovering gene function, but the paucity of available mutants affecting pollen development and/or function and the poor characterization of the known mutants have so far limited the exploitation of this approach. Here we present the cytological characterization ofgametophytic male sterile-1 (gaMS-1), a maize mutant that we identified in a program of transposon insertion mutagenesis for the production of mutations in gametophytically acting genes involved in microsporogenesis.gaMS-1 is expressed during or immediately after the first microspore division and leads to the production of immature, nonfunctional pollen grains. The mutation appears to affect the events leading to the developmental switch that follows the first microspore mitosis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02173101
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    Paper (German National Licenses)
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