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  • 1
    Electronic Resource
    Electronic Resource
    Specific arrangements of membrane particles at sites of exo-endocytosis in the freeze-etched neurohypophysis (1976)
    Springer
    Cell & tissue research 165 (1976), S. 317-325 
    Details
    ISSN: 1432-0878
    Keywords: Exocytosis, endocytosis ; Freeze-etching ; Electron microscopy ; Membrane particles, rosettes ; Neurohypophysis, neurosecretion ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Images have been obtained from freeze-etch replicas of neurohypophyses which are consistent with the view that orderly arranged aggregates of membrane particles occur in regions where fragments of membrane are being added to and taken away from the plasma membrane during secretion. Aggregates of particles included rosette-like and necklace-like patterns similar to those described by other authors at sites of exocytosis and endocytosis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00222436
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  • 2
    Electronic Resource
    Electronic Resource
    The influence of high pressure freezing on mammalian nerve tissue (1980)
    Springer
    Cell & tissue research 209 (1980), S. 201-216 
    Details
    ISSN: 1432-0878
    Keywords: High pressure (2,100 bars) freezing ; Freeze-etching ; Nerve tissue ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Vitrification of biological specimens in liquid nitrogen can be achieved under high pressure (2,100 bars). This procedure obviates the use of aldehyde fixation and cryoprotection (glycerol). The present work demonstrates its applicability to the freeze-etching of mammalian brain tissue. Freeze-fracture replicas from rat cerebellar cortex and subfornical organ prepared by this method are compared to conventionally processed material using aldehyde fixation, glycerination and freezing with Freon. The formation of large ice crystals is prevented in tissue blocks up to 0.5 mm thick; deep etching is markedly enhanced. Cytoplasmic microstructures such as mitochondrial cristae, microtubules and microfilaments, are readily observable against a finely granulated cytosol matrix. An additional advantage is the combined application with freeze-substitution.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00237626
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  • 3
    Electronic Resource
    Electronic Resource
    Neuritic growth cone and ependymal gap junctions in the feline subfornical organ during early development (1977)
    Springer
    Cell & tissue research 181 (1977), S. 27-36 
    Details
    ISSN: 1432-0878
    Keywords: Central nervous system (Subfornical organ) ; Gap junctions ; Neuritic growth cones ; Ependyma ; Electron microscopy ; Freezeetching
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Intercellular contacts in the subfornical organ (SFO) of kittens 3, 16, and 29 days old were studied in thin sections and by the freeze-etch method. Gap junctions appeared between growing nerve processes and target cells. The junctions were interspersed between immature synapses lacking mitochondria as well as full preand postsynaptic membrane specializations. Gap junctions were seen on filopodia as well as on more mature processes. The morphology of these junctions was typical of those described earlier but they were of small size (0.2–0.3 μm). Gap junctions of peculiar form were also seen between ependymal elements in the SFO at 16 days. These were of large size (0.5–0.8 μm) and were often of segmented character. This segmentation consisted of bands 3–4 particles in width with a center-to-center spacing of 90 nm with particle free corridors between corresponding to the width of about two rows of particles. The margin of the group might be circumscribed by a row of particles. Although gap junctions of large size were seen between ependymal cells in thin section, features corresponding to the particle free corridors have not been observed to date.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00222772
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