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  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Methods in cell science 22 (2000), S. 191-198 
    ISSN: 1573-0603
    Keywords: Canine ; Feline ; Flow Cytometry ; Gating ; Standardized
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Gating in flow cytometry is used to select subpopulations of cells for analysis. The technique is critical for subsequent analysis in order to select the population, free of debris and unrelated cells. Accurately quantifying subpopulations in clinical cases is necessary for correct diagnosis. Human lymphocytes are selected by backgating on populations of CD45+high CD14− cells. These reagents are not available widely across species. In veterinary medicine, markers to identify lymphocytes are usually limited to T-lymphocyte, CD4, CD8, and B-lymphocyte surface antigens. A standardized gating technique using a T-lymphocyte antibody is described and is applicable across species where limited phenotype markers are available.
    Type of Medium: Electronic Resource
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