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  • 1
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    Marine Fisheries Research Department, Southeast Asian Fisheries Development Center | Singapore
    In:  http://aquaticcommons.org/id/eprint/26829 | 23782 | 2019-11-19 01:08:33 | 26829 | Southeast Asian Fisheries Development Center, Marine Fisheries Research Department
    Publication Date: 2021-07-24
    Description: The paper provides the methodology in lipid extraction from fish using the Modified Folch's Method. Instructions on sample preparation, and the apparatus and reagents needed are presented. Detailed procedures in lipid extraction are provided.
    Keywords: Chemistry ; Fisheries ; Manuals ; Culture media ; Analysis ; Analytical techniques ; Methodology ; Chemical analysis ; Fish inspection ; Fishery industry ; Food technology ; Standards ; Specifications ; Lipids
    Repository Name: AquaDocs
    Type: book_section
    Format: application/pdf
    Format: application/pdf
    Format: C-2.1-C-2.2
    Format: 2
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  • 2
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    Marine Fisheries Research Department, Southeast Asian Fisheries Development Center | Singapore
    In:  http://aquaticcommons.org/id/eprint/26832 | 23782 | 2019-11-19 00:56:39 | 26832 | Southeast Asian Fisheries Development Center, Marine Fisheries Research Department
    Publication Date: 2021-07-24
    Description: The paper provides the methodology in determination of free fatty acids (FFA) in fish.
    Keywords: Chemistry ; Fisheries ; Manuals ; Culture media ; Analysis ; Analytical techniques ; Methodology ; Chemical analysis ; Food technology ; Standards ; Specifications ; Fatty acids
    Repository Name: AquaDocs
    Type: book_section
    Format: application/pdf
    Format: application/pdf
    Format: C-5.3
    Format: 1
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  • 3
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    Marine Fisheries Research Department, Southeast Asian Fisheries Development Center | Singapore
    In:  http://aquaticcommons.org/id/eprint/26838 | 23782 | 2019-11-19 00:36:53 | 26838 | Southeast Asian Fisheries Development Center, Marine Fisheries Research Department
    Publication Date: 2021-07-24
    Description: The paper provides the methodology in the preparation of methyl esters by boron trifluoride method in fish. Instructions on sample preparation and the apparatus and reagents needed are presented. Detailed procedures are also provided.
    Keywords: Chemistry ; Fisheries ; Manuals ; Culture media ; Analysis ; Analytical techniques ; Methodology ; Chemical analysis ; Food technology ; Standards ; Specifications ; Fish oils ; Fats ; Fatty acids ; Esters ; Lipids
    Repository Name: AquaDocs
    Type: book_section
    Format: application/pdf
    Format: application/pdf
    Format: C-11.1-C-11.2
    Format: 2
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  • 4
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    Marine Fisheries Research Department, Southeast Asian Fisheries Development Center | Singapore
    In:  http://aquaticcommons.org/id/eprint/26835 | 23782 | 2019-11-19 00:46:29 | 26835 | Southeast Asian Fisheries Development Center, Marine Fisheries Research Department
    Publication Date: 2021-07-24
    Description: The paper provides the methodology in the determination of thiobarbituric acid (TBA) number in fish fat. The apparatus and reagents needed are presented. Detailed procedures and calculations are also presented.
    Keywords: Chemistry ; Fisheries ; Manuals ; Culture media ; Analysis ; Analytical techniques ; Methodology ; Chemical analysis ; Food technology ; Standards ; Specifications ; Fish oils ; Fats ; Fatty acids
    Repository Name: AquaDocs
    Type: book_section
    Format: application/pdf
    Format: application/pdf
    Format: C-8.1-C-8.3
    Format: 3
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  • 5
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    Marine Fisheries Research Department, Southeast Asian Fisheries Development Center | Singapore
    In:  http://aquaticcommons.org/id/eprint/26837 | 23782 | 2019-11-19 00:39:35 | 26837 | Southeast Asian Fisheries Development Center, Marine Fisheries Research Department
    Publication Date: 2021-07-24
    Description: The paper provides the methodology in the determination of the degree of lipid oxidation by gas chromatography. Instructions on sample preparation and the detailed procedures and calculation are provided.
    Keywords: Chemistry ; Fisheries ; Manuals ; Culture media ; Analysis ; Analytical techniques ; Methodology ; Chemical analysis ; Food technology ; Standards ; Specifications ; Lipids ; Fats ; Fatty acids ; Oxidation ; Chromatographic techniques
    Repository Name: AquaDocs
    Type: book_section
    Format: application/pdf
    Format: application/pdf
    Format: C-10.1
    Format: 1
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  • 6
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    Marine Fisheries Research Department, Southeast Asian Fisheries Development Center | Singapore
    In:  http://aquaticcommons.org/id/eprint/26705 | 23782 | 2019-08-29 08:02:13 | 26705 | Southeast Asian Fisheries Development Center, Marine Fisheries Research Department
    Publication Date: 2021-07-24
    Description: Lizardfish, Saurida tumbil, is an abundant resource that is relatively underutilized. It is a potential raw material for surimi processing. Characterized by its ability to produce formaldehyde endogenously after catch, the lizardfish tends to have comparatively higher levels of formaldehyde, which is known to accelerate protein denaturation. As such minced meat from lizardfish has to be treated to enhance its gel-forming ability. The objective of this project is to improve the gel-forming ability of surimi made from fresh and frozen lizardfish by sodium pyrophosphate leaching (PL), and the use of egg white and beef-plasma protein concentrate. This was compared against the usual leaching (UL) method. Pyrophosphate leaching (PL) resulted in better gel-forming ability. Two-times of leaching using 0.2% sodium pyrophosphate resulted in the production of reasonably good quality fish jelly products from fresh and frozen lizardfish. Both egg white and beef-plasma protein concentrate improved the gel-forming ability of surimi from lizardfish. However, if the frozen raw material is of poor quality, neither pyrophosphate leaching nor the addition of egg-white or beef-plasma could improve the gel-forming ability of the surimi. The critical control point for raw material quality is its formaldehyde level. The best quality surimi is obtained when the formaldehyde level is below 15 ppm.
    Keywords: Fisheries ; Saurida tumbil ; Minced products ; Processing fishery products ; Experimental research
    Repository Name: AquaDocs
    Type: book_section
    Format: application/pdf
    Format: application/pdf
    Format: 183-199
    Format: 17
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  • 7
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    Marine Fisheries Research Department, Southeast Asian Fisheries Development Center | Singapore
    In:  http://aquaticcommons.org/id/eprint/26704 | 23782 | 2019-08-29 08:08:26 | 26704 | Southeast Asian Fisheries Development Center, Marine Fisheries Research Department
    Publication Date: 2021-07-24
    Description: The colour of tuna is the main criterion for quality assessment of sashimi. In Japan, the general rule is the brighter the red, the better the quality. The colour of fresh meat is due to the occurrence of myoglobin in its different chemical states; in oxidized meat, the predominant chemical state is metmyolgobin. In this study, the main objective is to correlate the amount of myoglobin with the quality of yellowfin tuna (Thunnus albacares). The tuna samples in this study were subjected to 0°C storage and their colour and myoglobin values were monitored over 14 days. The value of met-myoglobin for 0-day is 10.0% and on the 14th day, the value increased significantly to 95.9%. For chemical tests, significant spoilage occurred during the time between the 9th and 12th day of storage when met-myoglobin values increased from 67.5% to 83.4%. For sensory evaluation, all panelists rejected the tuna samples as unsuitable for sashimi when the value of met-myoglobin increased to 70%.
    Keywords: Fisheries ; Thunnus albacares ; Sashimi ; Colour ; Quality ; Evaluation
    Repository Name: AquaDocs
    Type: book_section
    Format: application/pdf
    Format: application/pdf
    Format: 172-182
    Format: 11
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  • 8
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    Marine Fisheries Research Department, Southeast Asian Fisheries Development Center | Singapore
    In:  http://aquaticcommons.org/id/eprint/26744 | 23782 | 2019-09-13 00:44:22 | 26744 | Southeast Asian Fisheries Development Center, Marine Fisheries Research Department
    Publication Date: 2021-07-24
    Description: Boiled, dried anchovies sold in Singapore are mainly imported from neighbouring countries. A survey was conducted to obtain an understanding of the quality of this product which is sold packed in polypropylene bags at supermarkets. The survey showed that the product had a high moisture content (=25% dry wt) and hence should be categorised as an intermediate-moisture product rather than as a dried product. It also had a high percentage breakage, a considerable amount of foreign matter, high water activity from 0.663 to 0.700 and total lipid of 7.0 - 8.6%. The organoleptic quality of most samples was acceptable. This survey showed that the quality of this product at retail outlets could be improved. The following are suitable quality indicators: percentage breakage, protein content, salt content, ash and acid insoluble ash, but they need not be monitored in storage studies. The suitable quality indices for quality changes of this product were identified as moisture content, water activity, total volatile basic nitrogen, total lipid, peroxide value, thiobarbituric acid number, colour and sensory evaluation. Trimethylamine nitrogen is, however not a suitable parameter. The effect of storage at different conditions (refrigerated: 7±2 C, 80% RH; room temperature exposed to light and kept in the dark: 27±40°C, 73±16% RH) on the shelflife of the product was also investigated. The samples were again found to have a high moisture and hence mould growth was the main contributor to spoilage at room temperature. Refrigerated storage extended the shelflife beyond six weeks, yielding a fairly good quality product. However it caused the moisture and water activity to increase with storage. The shelflife of samples stored at room temperature exposed to light was 4 weeks and those in the dark was two weeks. Drying and vacuum packing were done to study their effect on shelflife extension. The samples were further dried to a moisture of =25% (dry weight) using the oven and low temperature vacuum dryer, then vacuum packed and stored in an air-conditioned room. These were compared with two other samples that were not further dried; one lot was stored exposed at room temperature and the other was vacuum packed and stored in an air-conditioned room. The samples were monitored weekly for the first 6 weeks and thereafter monthly. The first 6 weeks of this experiment is reported here; these preliminary results showed that further drying and vacuum packaging effectively reduced the moisture and also retarded mould growth. Thus the product is still fairly good even after six weeks.
    Keywords: Fisheries ; Dried products ; Water content ; Quality control ; Storage conditions ; Storage life ; Evaluation ; Packing fishery products
    Repository Name: AquaDocs
    Type: book_section
    Format: application/pdf
    Format: application/pdf
    Format: 258-272
    Format: 15
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  • 9
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    Marine Fisheries Research Department, Southeast Asian Fisheries Development Center | Singapore
    In:  http://aquaticcommons.org/id/eprint/26836 | 23782 | 2019-11-19 00:43:28 | 26836 | Southeast Asian Fisheries Development Center, Marine Fisheries Research Department
    Publication Date: 2021-07-24
    Description: The paper provides the methodology of the determination of methyl esters of fatty acids by gas chromatographic method. Methyl esters of fatty acids from fish and animal fats having 8-24 carbon atoms are separated and determined by gas chromatography. The apparatus, reagents and operating conditions are presented. Detailed procedures of the analysis of the sample are also provided.
    Keywords: Chemistry ; Fisheries ; Manuals ; Culture media ; Analysis ; Analytical techniques ; Methodology ; Chemical analysis ; Food technology ; Standards ; Specifications ; Fish oils ; Fatty acids ; Fats
    Repository Name: AquaDocs
    Type: book_section
    Format: application/pdf
    Format: application/pdf
    Format: C-9.1-C-9.3
    Format: 3
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  • 10
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    Marine Fisheries Research Department, Southeast Asian Fisheries Development Center | Singapore
    In:  http://aquaticcommons.org/id/eprint/26827 | 23782 | 2019-11-19 01:11:10 | 26827 | Southeast Asian Fisheries Development Center, Marine Fisheries Research Department
    Publication Date: 2021-07-24
    Description: The paper discusses the significance of lipid analysis in fish. Fish lipids exist as phospholipids and triglycerides which deteriorate during storage due to hydrolysis and oxidation.
    Keywords: Chemistry ; Fisheries ; Manuals ; Culture media ; Analysis ; Analytical techniques ; Methodology ; Chemical analysis ; Lipids ; Organic compounds ; Fats ; Hydrolysis ; Chemical reactions ; Chemical degradation ; Oxidation
    Repository Name: AquaDocs
    Type: book_section
    Format: application/pdf
    Format: application/pdf
    Format: C-1.1
    Format: 1
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