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  • Fisheries  (5)
  • Cell & Developmental Biology  (2)
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  • 1
    Digitale Medien
    Digitale Medien
    New York, N.Y. : Wiley-Blackwell
    Journal of Cellular Biochemistry 19 (1982), S. 305-313 
    ISSN: 0730-2312
    Schlagwort(e): anti-iodiotypic antibody ; thyrotropin ; receptor ; thyroid stimulating antibody ; Graves disease ; Life and Medical Sciences ; Cell & Developmental Biology
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Biologie , Chemie und Pharmazie , Medizin
    Notizen: We raised an antihuman thyrotropin anti-idiotypic antibody and showed that it was active at the thyrotropin receptor. Thus this antibody inhibited 125I b-TSH binding to thyroid plasma membranes, stimulated adenylate cyclase activity through a guanyl nucleotide-dependent mechanism, increased radioiodide entry rate into isolated porcine thyroid follicular cells, and induced such cultured cells to organize into follicles. All these parameters are typical of thyrotropin action. This work raises the possibility that thyroid stimulating antibodies that cause the hyperthyroidism of Graves disease may be, at least in some patients, anti-thyrotropin anti-idiotypic antibodies. It also offers a novel method whereby antireceptor antibodies used in the isolation and characterization of the receptor may be raised from ligands.
    Zusätzliches Material: 5 Ill.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 2
    Digitale Medien
    Digitale Medien
    New York, N.Y. : Wiley-Blackwell
    Journal of Cellular Biochemistry 34 (1987), S. 151-162 
    ISSN: 0730-2312
    Schlagwort(e): anti-idiotypic antibodies ; thyrotropin subunits ; thyrotropin receptor ; monoclonal antibody ; Life and Medical Sciences ; Cell & Developmental Biology
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Biologie , Chemie und Pharmazie , Medizin
    Notizen: TSH is a heterodimeric glycoprotein hormone, whose dissociated subunits are without biological activity. This has precluded the assessment of the relative contribution of each subunit to hormone action. We have raised anti-idiotypes to monoclonal antibodies specific, respectively, for the α and β hTSH subunits. The anti-β anti-idiotype inhibited l25I-hTSH binding to the β subunit-specific monoclonal quantitatively, whereas 125I-hTSH binding to the α subunit-specific monoclonal was not inhibited by anti-α anti-idiotypes, suggesting that only the former is an “internal image” anti-idiotype. Neither of the two anti-idiotypes nor equimolar mixtures thereof inhibited 125I-bTSH binding to thyroid membranes, even though radiolabelled anti-idiotypes showed saturable binding to thyroid plasma membrane which was inhibited 41-65% by bTSH. Each anti-idiotype alone caused 9% inhibition (compared to 50% by NRIgG) of thyroid plasma membrane adenylate cyclase. Equimolar mixtures (125 μg/ml IgG of each anti-idiotype) induced enzyme activity equivalent to 85% of that of 250 mU/ml of TSH. The TSH-like action of the two anti-idiotypes was also reflected in their capacity to increase (450% by 250 μg/ml IgG compared to normal rabbit IgG) the uptake of 131I into isolated thyrocytes and to promote the organization of such cells into follicular structures. At 250 μg/ml, anti-β anti-idiotype promoted the organization of small follicles and only at a concentration of 500 μg/ml did it enhance 131I uptake. Anti α idiotype was without effect in both assays. Lastly, mixture of anti-idiotypes bound to the ∼ 197,000 Mr band (TSH holoreceptor) on protein blots of thyroid plasma membranes resolved on NaDSO4-polyacrylamide gel electrophoresis under non-reducing conditions. Individual anti-idiotypes were without effect.The TSH α and β subunits apparently deliver two cooperative signals to the receptor and that specificity is associated with the β subunit, while the α subunit is important in enhancing receptor affinity for the heterodimer and in stablizing TSH-receptor complex.
    Zusätzliches Material: 6 Ill.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 3
    facet.materialart.
    Unbekannt
    In:  http://aquaticcommons.org/id/eprint/18864 | 12051 | 2015-11-26 12:25:29 | 18864 | Bangladesh Fisheries Research Institute
    Publikationsdatum: 2021-07-12
    Beschreibung: The study was conducted in collaboration with the ECFC project of the FAO (BGD/97/017) in Cox's Bazar to develop a low cost solar tunnel dryer for the production of high quality marine dried fish. The study areas were Kutubdiapara, Maheshkhali and Shahparirdip under Cox's Bazar district. Three different models of low cost solar dryer were constructed with locally available materials such as bamboo, wood, bamboo mat, hemp, canvas, wire, nails, rope, tin, polythene and net. Size of the dryers were: 20x4x3 ft ; 30x3x3 ft and 65x3x3 ft with the costs of Tk. 3060, 3530, 9600 for dryer 1, 2 and 3, respectively having different models. The drying capacities were 50, 150, 500 kg for dryer 1, 2 and 3 respectively. The average temperature range inside the dryers were 29-43°C, 34-51°C and 37-57°C for dryer 1, 2 and 3 respectively as recorded at 8:30h to 16:30h. The relative humidity were in the ranges of 22-42%, 27-39% and 24-41 % in dryer 1, 2 and 3 respectively. The fish samples used were Bombay duck, Silver Jew fish and Ribbon fish. The total drying time was in the range of 30-42, 28-38 and 24-34 hours to reach the moisture content of 12.3-14.5, 11.8-14.3, and 11.6-14.1% in dryer 1, 2 and 3 respectively. Among these three fish samples the drying was faster in Silver Jew fish followed by Bombay duck and Ribbon fish in all the three dryer.
    Schlagwort(e): Fisheries ; design ; construction ; solar dryier ; temperature ; humidity
    Repository-Name: AquaDocs
    Materialart: article
    Format: application/pdf
    Format: application/pdf
    Format: 51-61
    Standort Signatur Erwartet Verfügbarkeit
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  • 4
    facet.materialart.
    Unbekannt
    In:  http://aquaticcommons.org/id/eprint/17714 | 9 | 2015-09-11 14:43:20 | 17714 | Bangladesh Fisheries Research Institute
    Publikationsdatum: 2021-06-27
    Beschreibung: An investigation was carried out to observe histopathological changes in liver and kidneyof suspected epizootic ulcerative syndrome (EUS)-affected shing fish, Heteropneustesfossilis (Bloch) collected from the "Agro-3 fish farm" situated at Boilor, Trishal,Mymensingh. Focal necrosis, haemorrhages and atrophy of the sinusoidal region wereobserved in the liver tissue. Fungal granulomas were found both in liver and kidney. Insome cases fatty depositions were observed in all over the hepatic tissue. Degenerationand necrosis of renal tubular epithelial cells were also occurred. Missing of glomerulusand necrosis surrounding the Bowman's capsule in the kidney tissue were found.
    Schlagwort(e): Biology ; Fisheries ; Heteropneustes fossilis ; EUS ; Histopathology
    Repository-Name: AquaDocs
    Materialart: article
    Format: application/pdf
    Format: application/pdf
    Format: 21-26
    Standort Signatur Erwartet Verfügbarkeit
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  • 5
    facet.materialart.
    Unbekannt
    In:  http://aquaticcommons.org/id/eprint/17723 | 9 | 2015-09-11 14:43:39 | 17723 | Bangladesh Fisheries Research Institute
    Publikationsdatum: 2021-06-28
    Beschreibung: Pathogenicity of Aeromonas hydrophila bacteria was tested on the stinging catfishHeteropneustes fossilis. Before artificial infection the morphological, biochemical andphysiological characters of Aeromonas hydrophila were studied. The infections were doneby two different methods, viz., intramuscular (IM) and intraperitoneal (IP) injection. Ininfection experiment, each group of 10 fish were injected either intramuscularly orintra peritoneally with one dose higher than the LD50 dose (9.6 x 107 CFU/fish). All thefish tested died within 1 to 9 days. Both in cases of intramuscular and intraperitonealinjection, external pathology were found. Haemorrhagic lesions were evident at the siteof injection. The posterior end of the body surface was found to develop greyish-whitelesion that was extended up to caudal fin. Hyperemic anal region and the fin bases werealso observed. Total bacterial loads in liver, kidney and intestine were determined.Aeromonas hydrophila could be isolated from liver, kidney and intestine of theexperimentally infected fish. In case of intramuscular injection the highest and thelowest bacterial load was found to be 2.4 x 107 CFU/g of liver and 2.1 x 102 CFU/g ofkidney and in case of intraperitoneal injection they were found to be 3.6 x 106 CFU/g ofkidney and 1.2 x 104 CFU/g of kidney respectively. It was concluded that A. hydrophilacould cause serious disease condition to Heteropneustes fossilis and its pathogenesis in thefish was also very efficient.
    Schlagwort(e): Biology ; Fisheries ; Experimental pathogenesis ; Heteropneustes fossilis ; Aeromonas hydrophila
    Repository-Name: AquaDocs
    Materialart: article
    Format: application/pdf
    Format: application/pdf
    Format: 27-33
    Standort Signatur Erwartet Verfügbarkeit
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  • 6
    facet.materialart.
    Unbekannt
    In:  http://aquaticcommons.org/id/eprint/17789 | 123 | 2015-09-13 05:25:44 | 17789 | Bangladesh Fisheries Research Institute
    Publikationsdatum: 2021-06-29
    Beschreibung: The study was conducted to determine the cost, return and relative profitability ofpond fish production of Mymensingh and Jessore districts. A total of 75 ponds wereselected on the basis of purposive random sampling technique from 7 villages under 2Upazila (Trishal and Gouripur) of Mymensingh districts and 8 villages under 4Upazila (Monimmpur, Jhikorgacha, Chowgacha and Sadar) of Jessore district. It wasfound that per hectare per year gross cost of pond fish production in Mymensingh andJessore were Tk 333457.75 and Tk 54327.74, while gross return were Tk 434131.16 andTk. 96640.00 and net return were Tk 100673.41 and Tk. 42312.26, respectively. Thefindings of this study revealed that the pond fish production in Jessore district wasmore profitable than that of Mymensingh district. Cobb-Douglas production functionwas applied to realize the specific effect of the factors on pond fish production. Out ofsix variables included in the function three variables had positive impact on returnfrom pond fish production, in Mymensingh district but five variables had positiveimpact on return from pond fish production in Jessore district
    Schlagwort(e): Aquaculture ; Fisheries ; Fish culture ; Cost and return ; Cob-Duglas production function
    Repository-Name: AquaDocs
    Materialart: article
    Format: application/pdf
    Format: application/pdf
    Format: 151-156
    Standort Signatur Erwartet Verfügbarkeit
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  • 7
    facet.materialart.
    Unbekannt
    In:  http://aquaticcommons.org/id/eprint/17791 | 17292 | 2015-09-13 10:15:01 | 17791 | Bangladesh Fisheries Research Institute
    Publikationsdatum: 2021-06-29
    Beschreibung: The paper examines the factor intensity and economic returns of alternate shrimp-crop and shrimp-salt farming in the coastal areas of Bangladesh. Data were collected from 30 shrimp-crop and 30 shrimp-salt farmers, 30 shrimp farmers and 30 rice farmers from three selected coastal districts of Bangladesh. Cobb-Douglas production function model was used to determine the effect of various factors on alternate shrimp-crop farming. The chosen variables were stocking of juveniles, paddy seed, labour, fertilizers, feed and farm size of respective type of farming. The results indicated that the production function exhibited increasing remrns to scale for alternate shrimp-rice, alternate shrimp-salt and year round shrimp farming while it indicated decreasing returns for year round rice farming. Economic analysis of same system of farming indicated that higher amount of input use produced higher level of yield, gross return and net return for each type of production system.
    Schlagwort(e): Biology ; Fisheries ; Economic return ; Alternate shrimp-crop farming
    Repository-Name: AquaDocs
    Materialart: article
    Format: application/pdf
    Format: application/pdf
    Format: 179-188
    Standort Signatur Erwartet Verfügbarkeit
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