ALBERT

All Library Books, journals and Electronic Records Telegrafenberg

X
feed icon rss

Your email was sent successfully. Check your inbox.

An error occurred while sending the email. Please try again.

Proceed reservation?

Export
Filter
  • Fish developmental biology  (1)
  • Life and Medical Sciences  (1)
  • 1990-1994  (2)
Collection
Keywords
Publisher
Years
Year
  • 1
    Electronic Resource
    Electronic Resource
    Transient expression of foreign DNA during embryonic and larval development of the medaka fish (Oryzias latipes) (1991)
    Springer
    Molecular genetics and genomics 226 (1991), S. 129-140 
    Details
    ISSN: 1617-4623
    Keywords: Medaka ; Gene transfer ; Transient expression ; DNA fate ; Fish developmental biology
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Species of small fish are becoming useful tools for studies on vertebrate development. We have investigated the developing embryo of the Japanese medaka for its application as a transient expression system for the in vivo analysis of gene regulation and function. The temporal and spatial expression patterns of bacterial chloramphenicol acetyltransferase and galactosidase reporter genes injected in supercoiled plasmid form into the cytoplasm of one cell of the two-cell stage embryo was promoter-specific. The transient expression was found to be mosaic within the tissue and organs reflecting the unequal distribution of extrachromosomal foreign DNA and the intensive cell mixing movements that occur in fish embryogenesis. The expression data are consistent with data on DNA fate. Foreign DNA persisted during embryogenesis and was still detectable in some 3- and 9-month-old adult fish; it was found in high molecular weight form as well as in circular plasmid conformations. The DNA was replicated during early and late embryogenesis. Our data indicate that the developing medaka embryo is a powerful in vivo assay system for studies of gene regulation and function.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00273596
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 2
    Electronic Resource
    Electronic Resource
    Methodik und Anwendungsgebiete des genetischen Fingerabdruckverfahrens (1994)
    Weinheim : Wiley-Blackwell
    Biologie in unserer Zeit 24 (1994), S. 9-14 
    Details
    ISSN: 0045-205X
    Keywords: Life and Medical Sciences
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: Unter einem genetischen Fingerabdruck („DNA-Fingerprint“) versteht man ein mit molekularbiologischen Methoden erstelltes Bandenmuster aus informationsfreien DNA-Abschnitten, welches hochspezifisch für das entsprechende Individuum ist. Die Entdeckung und Weiterentwicklung des genetischen Fingerabdrucks hat innerhalb kürzester Zeit zu erheblichen Fortschritten in den verschiedenen Teildisziplinen der Biologie und Medizin geführt. Durch das Aufzeigen individueller Unterschiede im Erbgut werden mit dieser Methode Grundlagen für die Bearbeitung zahlreicher Fragestellungen geschaffen. Wir wollen in diesem Aufsatz zum einen die Methodik veranschaulichen und zum anderen einige ausgewählte Anwendungsbeispiele vorstellen, die verdeutlichen sollen, welches Potential sich hinter der Methode des „DNA-Fingerprinting“ verbirgt.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/biuz.19940240103
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
Close ⊗
This website uses cookies and the analysis tool Matomo. More information can be found here...