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  • FLUID MECHANICS AND HEAT TRANSFER  (5)
  • SPACE SCIENCES  (5)
  • Biochemistry and Biotechnology  (4)
  • 1
    Electronic Resource
    Electronic Resource
    On-line monitoring and control of methanol concentration in shake-flask cultures of Pichia pastoris (1997)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 56 (1997), S. 279-286 
    Details
    ISSN: 0006-3592
    Keywords: methanol sensor ; methanol monitoring and control ; methylotrophic yeast fermentation ; Pichia pastoris ; transferrin ; shake-flask cultures ; Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: The methylotrophic yeast Pichia pastoris can be used to express recombinant genes at high levels under the control of the methanol-inducible alcohol oxidase 1 (AOX1) promoter. Accurate regulation of the methanol concentration in P. pastoris cultures is necessary to maintain induction, while preventing accumulation of methanol to cytotoxic levels. We developed an inexpensive methanol sensor that uses a gas-permeable silicone rubber tube immersed in the culture medium and an organic solvent vapor detector. The sensor was used to monitor methanol concentration continuously throughout a fed-batch shake-flask culture of a P. pastoris clone producing the N-lobe of human transferrin. The sensor calibration was stable for the duration of the culture and the output signal accurately reflected the methanol concentration determined off-line by HPLC. A closed-loop control system utilizing this sensor was developed and used to maintain a 0.3% (v/v) methanol concentration in the culture. Use of this system resulted in a fivefold increase in volumetric protein productivity over levels obtained using the conventional fed-batch protocol. © 1997 John Wiley & Sons, Inc. Biotechnol Bioeng 56: 279-286, 1997.
    Additional Material: 7 Ill.
    Type of Medium: Electronic Resource
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    Paper (German National Licenses)
  • 2
    Electronic Resource
    Electronic Resource
    Bacterial adsorption to smooth surfaces: Rate, extent, and spatial pattern (1985)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 27 (1985), S. 1662-1667 
    Details
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: The influence of bulk-water bacterial cell concentration and specific growth rate history on bacterial adsorption rates to surfaces was investigated using response surface analysis. A pure culture of Pseudomonas sp. 224S was grown in a chemostat and pumped into a continuous flow reactor where the bacteria were exposed to clean, glass surfaces under turbulent flow conditions for a period of six hours. Adsorption rate decreased approximately linearly with increasing specific growth rate history. Glass surfaces became saturated with 224S at ca. 0.1% coverage and the resulting spatial pattern of the adsorbed cells deviated from random in the direction of uniformity.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bit.260271206
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Microbial desulfurization of coal by thermophilic microorganism Sulfolobus acidocaldarius (1982)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 24 (1982), S. 2115-2121 
    Details
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bit.260240919
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  • 4
    Electronic Resource
    Electronic Resource
    Activity of Pseudomonas aeruginosa in biofilms: Steady state (1984)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 26 (1984), S. 1418-1424 
    Details
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Aerobic glucose metabolism by Pseudomonas aeruginosa in steady-state biofilms at various substrate loading rates and reactor dilution rates was investigated. Variables monitored were substrate (glucose), biofilm cellular density, biofilm extracellular polymeric substance (EPS) density, and suspended cellular and EPS concentrations. A mathematical model developed to describe the system was compared to experimental data. Intrinsic yield and rate coefficients included in the model were obtained from suspended continuous culture studies of glucose metabolism by P. aeruginosa. Experimental data compared well with the mathematical model, suggesting that P. aeruginosa does not behave differently in steady-state biofilm cultures, where diffusional resistance is negligible, than in suspended cultures. This implies that kinetic and stoichiometric coefficients for P. aeruginosa derived in suspended continuous culture can be used to describe steady-state biofilm processes.
    Additional Material: 7 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bit.260261204
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  • 5
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    Ground-based photography of the Mariner IV REGION of Mars. (1966)
    In:  Other Sources
    Details
    Publication Date: 2019-05-30
    Description: Data from ground photography of Mars covering portions of Mariner IV path, noting position of oases and canals
    Keywords: SPACE SCIENCES
    Type: ICARUS
    Format: text
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    NASA TECHNICAL REPORTS
  • 6
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    Some aspects of algorithm performance and modeling in transient thermal analysis of structures (1982)
    In:  CASI
    Details
    Publication Date: 2016-06-07
    Description: The status of an effort to increase the efficiency of calculating transient temperature fields in complex aerospace vehicle structures is described. The advantages and disadvantages of explicit and implicit algorithms are discussed. A promising set of implicit algorithms with variable time steps, known as the GEAR package is described. Four test problems, used for evaluating and comparing various algorithms, were selected and finite element models of the configurations are described. These problems include a space shuttle frame component, an insulated cylinder, a metallic panel for a thermal protection system, and a model of the space shuttle orbiter wing. Results generally indicate a preference for implicit oer explicit algorithms for solution of transient structural heat transfer problems when the governing equations are stiff.
    Keywords: FLUID MECHANICS AND HEAT TRANSFER
    Type: Computational Aspects of Heat Transfer in Struct.; p 91-98
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  • 7
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    The SPAR thermal analyzer: Present and future (1982)
    In:  CASI
    Details
    Publication Date: 2016-06-07
    Description: The SPAR thermal analyzer, a system of finite-element processors for performing steady-state and transient thermal analyses, is described. The processors communicate with each other through the SPAR random access data base. As each processor is executed, all pertinent source data is extracted from the data base and results are stored in the data base. Steady state temperature distributions are determined by a direct solution method for linear problems and a modified Newton-Raphson method for nonlinear problems. An explicit and several implicit methods are available for the solution of transient heat transfer problems. Finite element plotting capability is available for model checkout and verification.
    Keywords: FLUID MECHANICS AND HEAT TRANSFER
    Type: Computational Aspects of Heat Transfer in Struct.; p 35-50
    Format: application/pdf
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  • 8
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    Evaluation of the SPAR thermal analyzer on the CYBER-203 computer (1982)
    In:  CASI
    Details
    Publication Date: 2016-06-07
    Description: The use of the CYBER 203 vector computer for thermal analysis is investigated. Strengths of the CYBER 203 include the ability to perform, in vector mode using a 64 bit word, 50 million floating point operations per second (MFLOPS) for addition and subtraction, 25 MFLOPS for multiplication and 12.5 MFLOPS for division. The speed of scalar operation is comparable to that of a CDC 7600 and is some 2 to 3 times faster than Langley's CYBER 175s. The CYBER 203 has 1,048,576 64-bit words of real memory with an 80 nanosecond (nsec) access time. Memory is bit addressable and provides single error correction, double error detection (SECDED) capability. The virtual memory capability handles data in either 512 or 65,536 word pages. The machine has 256 registers with a 40 nsec access time. The weaknesses of the CYBER 203 include the amount of vector operation overhead and some data storage limitations. In vector operations there is a considerable amount of time before a single result is produced so that vector calculation speed is slower than scalar operation for short vectors.
    Keywords: FLUID MECHANICS AND HEAT TRANSFER
    Type: Computational Aspects of Heat Transfer in Struct.; p 405-424
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  • 9
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    Ground-based photography of the mariner iv region of mars (1965)
    In:  CASI
    Details
    Publication Date: 2019-05-24
    Description: Mars surface as seen by Mariner IV photographs
    Keywords: SPACE SCIENCES
    Type: NASA-CR-64466 , TN-701-66-10
    Format: application/pdf
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  • 10
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    Studies of implicit and explicit solution techniques in transient thermal analysis of structures (1982)
    In:  CASI
    Details
    Publication Date: 2019-06-28
    Description: Studies aimed at an increase in the efficiency of calculating transient temperature fields in complex aerospace vehicle structures are reported. The advantages and disadvantages of explicit and implicit algorithms are discussed and a promising set of implicit algorithms with variable time steps, known as GEARIB, is described. Test problems, used for evaluating and comparing various algorithms, are discussed and finite element models of the configurations are described. These problems include a coarse model of the Space Shuttle wing, an insulated frame tst article, a metallic panel for a thermal protection system, and detailed models of sections of the Space Shuttle wing. Results generally indicate a preference for implicit over explicit algorithms for transient structural heat transfer problems when the governing equations are stiff (typical of many practical problems such as insulated metal structures). The effects on algorithm performance of different models of an insulated cylinder are demonstrated. The stiffness of the problem is highly sensitive to modeling details and careful modeling can reduce the stiffness of the equations to the extent that explicit methods may become the best choice. Preliminary applications of a mixed implicit-explicit algorithm and operator splitting techniques for speeding up the solution of the algebraic equations are also described.
    Keywords: FLUID MECHANICS AND HEAT TRANSFER
    Type: NASA-TP-2038 , L-15245 , NAS 1.60:2038
    Format: application/pdf
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