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  • Euglena gracilis  (5)
  • Earth Resources and Remote Sensing  (2)
  • 1
    ISSN: 0014-5793
    Keywords: Chloroplast genome ; DNA fragment Eco-I ; Euglena gracilis ; Protein 32 00 M"r ; Restriction enzyme ; Thylakoid membrane
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology , Chemistry and Pharmacology , Physics
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1432-0983
    Keywords: Chloroplast DNA ; Euglena gracilis ; Origin of DNA replication
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary We sequenced a Bg1II-HindIII DNA fragment of the Euglena gracilis chloroplast genome which most likely carries the single origin of DNA replication according to recent electronmicroscopic mapping studies (Koller and Delius 1982a; Ravel-Chapuis et al. 1982). This DNA fragment contains a polymorphic region (Schlunegger et al. 1983) which is composed, as will be shown, of multiple tandem repeats (54 bp, 87% A+T). Furthermore we located on this DNA fragment a short inverted repeat element (96 positions) observed in the electronmicroscopic studies (Koller and Delius 1982b). Between the borders of the polymorphic region and the nearby inverted repeat (distance of 179 positions) we retrieved an exact copy of parts of the rDNA leader (105 positions) including 49 positions of the chloroplast trnW gene. A computer search for bacterial type Ori-regions did not reveal any significant sequence homology. However, the polymorphic region and its immediate vicinity have the capacity to form multiple stem and loop structures which may be involved in DNA replication initiation.
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 1432-0983
    Keywords: Euglena gracilis ; Chloroplast genome ; psb C ; Intron ORF
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary We retrieved a 1.6 kbp intron separating two exons of the psb C gene which codes for the 44 kDa reaction center protein of photosystem II. This intron is 3 to 4 times the size of all previously sequenced Euglena gracilis chloroplast introns. It contains an open reading frame of 458 codons potentially coding for a basic protein of 54 kDa of yet unknown function. The intron boundaries follow consensus sequences established for chloroplast introns related to class II and nuclear pre-mRNA introns. Its 3′-terminal segment has structural features similar to class II mitochondrial introns with an invariant base A as possible branch point for lariat formation.
    Type of Medium: Electronic Resource
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  • 4
    ISSN: 1432-0983
    Keywords: Chloroplast DNA ; Euglena gracilis ; rrn operons ; DNA deletion/insertion
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary We have completed the analysis of a DNA segment of the chloroplast genome of Euglena gracilis Klebs, Z-strain, which links the 3′ end of the extra 16S rRNA gene with the 5′ end of the 16S rRNA gene of the rrn operon A. This region is a mosaic of several structural elements and contains an intact rrn interoperon spacer of 1,080 bp, an extra 5S rRNA gene, an open reading frame for 406 codons (ORF 406) which is flanked by short inverted repeats and a short direct repeat originating from the rrn interoperon spacer. It seems that a once complete rrn operon underwent in the past an insertion/deletion event leaving intact the 16S and 5S rRNA but totally excising the 16S-23S intergenic spacer and the 23S rRNA gene. Instead a protein coding gene of yet unknown function was inserted along with other structural elements.
    Type of Medium: Electronic Resource
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  • 5
    ISSN: 1573-5028
    Keywords: chloroplast ; Euglena gracilis ; ORF406 ; rRNA-mRNA co-transcript ; supplementary 16 S rRNA
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The chloroplast genome of Euglena gracilis contains a supplementary gene for a 16 S rRNA (s16 S rrn gene), which is not part of a complete rrn operon. An open reading frame (ORF406) is located downstream of the s16 S rrn gene. Chloroplast RNA was hybridized with cloned DNA fragments of this region and the hybrids were analysed by electron microscopy and S1-nuclease protection experiments. The s16 S rrn gene and the ORF406 are transcribed as one continuous 3.6 kb long RNA, which starts just upstream of the 5′-end of the s16 S rrn gene. The 3′-end occurs at multiple sites within a region of 700 bases downstream of the ORF. Northern blot analysis shows that the abundance of the transcript is comparable with that of other chloroplast mRNAs.
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  • 6
    Publication Date: 2020-01-07
    Description: No abstract available
    Keywords: Earth Resources and Remote Sensing
    Type: JPL-CL-16-2915 , Workshop on Remote sensing in the O2 A-band; Jul 08, 2016; De Bilt; Netherlands
    Format: text
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  • 7
    Publication Date: 2019-07-10
    Description: This research note shows the results of applying a new massively parallel version of the automatic classification program (AutoClass IV) to a particular Landsat/TM image. The previous results for this image were produced using a "subsampling" technique because of the image size. The new massively parallel version of AutoClass allows the complete image to be classified without "subsampling", thus yielding improved results. The area in question is the FIFE study area in Kansas, and the classes AutoClass found show many interesting subtle variations in types of ground cover. Displays of the spatial distributions of these classes make up the bulk of this report. While the spatial distribution of some of these classes make their interpretation easy, most of the classes require detailed knowledge of the area for their full interpretation. We hope that some who receive this document can help us in understanding these classes. One of the motivations of this exercise was to test the new version of AutoClass (IV) that allows for correlation among the variables within a class. The scatter plots associated with the classes show that this correlation information is important in separating the classes. The fact that the spatial distribution of each of these classes is far from uniform, even though AutoClass was not given information about positions of pixels, shows that the classes are due to real differences in the image.
    Keywords: Earth Resources and Remote Sensing
    Type: FIA-94-01
    Format: text
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