ISSN:
1420-9071
Keywords:
Esterase
;
carboxylesterase
;
hydrolase
;
Myzus nicotianae
;
tobacco aphid
;
Myzus persicae
;
green peach aphid
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Medicine
Notes:
Abstract Naphthalene thioesters were synthesized as substrates for a continuous, non- disruptive kinetic assay of general carboxylesterase activity. The continuous nature of the assay is based on the production of a soluble dianion chromophore from the reaction of naphthalene thio with 5,5′-dithiobis (2-nitrobenzoic acid). Applications with 1- and 2-naphthalene thioacetates demonstrated their use in a fast accurate kinetic microassay of esterase activity, using porcine carboxylesterase as a model. These novel esters proved to be useful as substrates for the spectrophotometric assay of insecticide-resistance in two aphid species and may be applicable to other esterasebased diagnostic procedures.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF01955166
Permalink
