ISSN:
1617-4623
Keywords:
Key wordsPWP2
;
WD-repeat
;
β-Transducin
;
Essential gene
;
Yeast
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract WD-repeat proteins contain four to eight copies of a conserved motif that usually ends with a tryptophan-aspartate (WD) dipeptide. The Saccharomyces cerevisiae PWP2 gene, identified by sequencing of chromosome III, is predicted to contain eight so-called WD-repeats, flanked by nonhomologous extension. This gene is expressed as a 3.2-kb mRNA in all cell types and encodes a protein of 104 kDa. The PWP2 gene is essential for growth because spores carrying the PWP2Δ1::HIS3 disruption germinate before arresting growth with one or two large buds. The growth defect of pwp2Δ1::HIS3 cells was rscued by expression of PWP2 or epitope-tagged HA-PWP2 using the galactose-inducible GAL1 promoter. In the absence of galactose, depletion of Pwp2p resulted in multibudded cells with defects in bud site selection, cytokinesis, and hydrolysis of the septal junction between mother and daughter cells. In cell fractionation studies, HA-Pwp2p was localized in the particulate component of cell lysates, from which it would be solubulized by high salt and alkaline buffer but not by nonionic detergents or urea. Indirect immunofluorescence microscopy indicated that HA-Pwp2p was clustered at multiple points in the cytoplasm. These results suggest that Pwp2p exists in a proteinaceous complex, possibly associated with the cytoskeleton, wher it functions in control of cell growth and separation.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/s004389670012
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