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  • Escherichia coli  (2)
  • 1
    Electronic Resource
    Electronic Resource
    The isolation and preliminary characterisation of a novel Escherichia coli mutant rorB with enhanced sensitivity to ionising radiation (1988)
    Springer
    Molecular genetics and genomics 215 (1988), S. 161-164 
    Details
    ISSN: 1617-4623
    Keywords: Escherichia coli ; Ionising radiation ; Mutation ; DNA repair
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Escherichia coli K803 cells were mutagenized and screened for the presence of clones sensitive to ψ-rays but not to ultraviolet light. One new mutant of this type, named rorB, was isolated. This mutant is both cross-sensitive to mitomycin C and shows reduced conjugal recombination frequencies, but to a lesser extent than the phenotypically similar mutant recN. Unlike previously reported mutants of E. coli or yeast with an enhanced sensitivity to ionising radiations, rorB appears to be near wild type in ability to rejoin DNA double-strand breaks. The rorB gene maps close to ilvGEDAC at 84.5 min of the E. coli chromosome.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00331319
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    The cloning of the rorB gene of Escherichia coli (1988)
    Springer
    Molecular genetics and genomics 215 (1988), S. 156-160 
    Details
    ISSN: 1617-4623
    Keywords: Ionising radiation ; Gene cloning ; DNA repair ; Escherichia coli ; Mitomycin C
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A segment of the Escherichia coli genome which complements the ionising radiation sensitivity of the rorB mutation was cloned into pBR322. This DNA segment also complements the mitomycin C sensitivity of the rorB mutation. The gene was subcloned until defined in a fragment of 1.05 kb. Only one gene product, a protein of approximately 16.5 kDa, was found on maxicell analysis of the various subclones. Iso-electric focusing of this gene product suggests it may function in a complex.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00331318
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    Paper (German National Licenses)
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