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  • GEOPHYSICS  (6)
  • Engineering  (4)
  • Escherichia coli  (3)
  • 1985-1989  (13)
  • 1930-1934
  • 1
    ISSN: 1432-072X
    Schlagwort(e): Iron-limitation ; Escherichia coli ; Respiratory chains ; Cytochromes ; Gallium ; Metal uptake
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract The effects of iron limitation on growth, the composition and function of the respiratory chains, and gallium uptake inEscherichia coli have been studied. Decreasing the iron concentration in a defined medium using Chelex resin gave lower growth yields in both continuous culture and prolonged batch culture. In the former, ironlimited (entering [Fe]≤2.0 μM) cells exhibited diminished respiration rates, respiration-driven proton translocation quotients, and levels of non-haem iron and cytochromes. The cellular concentration of haemoproteinb-590 (a cytochromea 1-like hydroperoxidase) decreased 20-fold on iron limitation, whilst a CO-binding pigment with an absorption maximum in the dithionite-treated form near 500 nm appeared. Gallium(III) (9 μM) added to iron-limited, but not iron-sufficient, cultures diminished growth yields further; cells grown with low entering concentrations of iron took up less gallium than iron-sufficient cells. These results are attributed to the interference by gallium(III) with siderophore-mediated metal uptake. Gallium also stimulated iron uptake and was itself accumulated by iron-sufficient cells, suggesting that gallium(III) also affects the iron transport system(s) of low affinity.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 2
    ISSN: 1432-072X
    Schlagwort(e): Copper-limitation ; Escherichia coli ; Cytochrome oxidases ; Oxygen reduction ; Respiratory chains
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract The role(s) of copper in a bacterial cytochrome oxidase of the aa 3-type was investigated by growth of Paracoccus denitrificans NCIB 8944, in batch and steady state continuous culture, in a medium from which the bulk of the copper had been extracted. In a medium containing approximately 0.02 μM copper, cellular copper content, cytochromes a+a 3 and cytochrome a 3 were reduced to 55%, 58% and 33% respectively of control values and there were also less marked decreases in cytochromes c+c 1 (to 85%) and a CO-binding b-type cytochrome, possibly cytochrome o (to 71%). Copper deficiency elicited in reduced minus oxidized difference spectra a shift to shorter wavelengths and narrowing of the band width of the α-band of the oxidase, and loss of a (negative) band near 830 nm attributable to CuA (the copper functionally associated with haem a in the oxidase complex). The oxidase in copper-deficient cells reacted with oxygen to form the oxy “Compound A” at rates similar to that in control cells but CO recombination to ferrous haem a 3 was slowed 4-fold in the copper deficient case. The results are interpreted as indicating loss of CuA and changes in the proportions of haems a and a 3 with retention of catalytic activity. Titrations of respiration rates with antimycin suggested that copper deficiency did not result in diversion of electron flux through an antimycin A-insensitive, cytochrome o-terminated branch of the respiratory chain.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 3
    Digitale Medien
    Digitale Medien
    Springer
    Molecular genetics and genomics 213 (1988), S. 99-104 
    ISSN: 1617-4623
    Schlagwort(e): Promoters ; Escherichia coli ; Proteus vulgaris ; Hemolysin
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary The hemolytic activity of Escherichia coli and Proteus vulgaris is determined by common contiguous genes encoding synthesis (hly C, hly A) and specific secretion (hly B, hly D) of active hemolysin. Nevertheless, the hlyC-proximal DNA sequences directing production of the homologous hemolysins by the recombinant DNAs P. vulgaris pVU763-709 and E. coli pANN202-312 showed no extensive homology. Primer extension and S1 nuclease protection were used to define in the two sequences the 5′ termini of hly transcripts synthesized in vivo and thus to infer the active hly promoters sequences. The E. coli hly C upstream region contained three separate promotors directing in vivo hly transcription, while the corresponding transcription of the P. vulgaris hly operon originated from a single distinct promotor, the-35 and-10 sequences of which formed part of an inverted repeat sequence. Elevated hemolytic activity caused by upstream Tn5 insertions in pVU763-709 resulted from increased transcription from this promotor.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 4
    Digitale Medien
    Digitale Medien
    Chichester [u.a.] : Wiley-Blackwell
    International Journal for Numerical Methods in Engineering 28 (1989), S. 1485-1485 
    ISSN: 0029-5981
    Schlagwort(e): Engineering ; Engineering General
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Mathematik , Technik allgemein
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 5
    Digitale Medien
    Digitale Medien
    Chichester [u.a.] : Wiley-Blackwell
    International Journal for Numerical Methods in Engineering 21 (1985), S. 163-168 
    ISSN: 0029-5981
    Schlagwort(e): Engineering ; Engineering General
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Mathematik , Technik allgemein
    Notizen: A fully discrete stability and accuracy analysis of some algorithms for the one-dimensional heat equation is presented. Results illustrate that 2-pass explicit schemes which simultaneously employ lumped and coupled capacity matrices are capable of improved performance over the standard 1-pass explicit scheme.
    Zusätzliches Material: 1 Ill.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 6
    ISSN: 0271-2091
    Schlagwort(e): Advection-diffusion equation ; Advective-diffusive systems ; Artificial-diffusion ; Compressible flows ; Discontinuous Galerkin method ; Entropy Error analysis ; Euler equations ; Finite elements ; Galerkin method ; Hyperbolic systems ; Incompressible flow ; Navier-Stokes equations ; Petrov-Galerkin method ; Space-time formulation ; Upwind methods ; Weighted residual methods ; Engineering ; Engineering General
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Maschinenbau
    Notizen: SUPG methods were originally developed for the scalar advection-diffusion equation and the incompressible Navier-Stokes equations. In the last few years successful extensions have been made to symmetric advective-diffusive systems and, in particular, the compressible Euler and Navier-Stokes equations. New procedures have been introduced to improve resolution of discontinuities and thin layers. In this paper a brief overview is presented of recent progress in the development and understanding of SUPG methods.
    Zusätzliches Material: 6 Ill.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 7
    ISSN: 0748-8025
    Schlagwort(e): Engineering ; Engineering General
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Mathematik , Technik allgemein
    Notizen: If bending moments converge at a sufficiently fast rate, it is proved that transverse shear stress resultants calculated from the moment equilibrium equation also converge. The result holds independent of the plate theory or type of element employed.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 8
    Publikationsdatum: 2011-08-19
    Beschreibung: This paper presents a representative example of an enhancement in energetic ion flux associated with the International Sun-Earth Explorer 3 (ISEE 3) spacecraft's encounter with a traveling compression region (TCR). Data from the energetic particle anisotropy spectrometer (EPAS) instrument on ISEE 3 are studied, along with magnetic field data from the vector helium magnetometer. It is concluded that the ion enhancements seen are spatial in nature, thus supporting the idea that TCRs are the lobe signatures of plasmoids moving along the magnetotail, away from earth.
    Schlagwort(e): GEOPHYSICS
    Materialart: Journal of Geophysical Research (ISSN 0148-0227); 92; 64-70
    Format: text
    Standort Signatur Erwartet Verfügbarkeit
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  • 9
    Publikationsdatum: 2011-08-19
    Beschreibung: Magnetotail observations from the ISEE 3 distant (1983) tail mission taken during the Coordinated Data Analysis Workshop 8 (CDAW 8) A and G events are investigated. The ISEE 3 magnetic field, plasma, and energetic particle measurements taken in these two plasmoids have been analyzed and compared with various equilibrium structures and propagating waves/tail oscillation modes. Results indicate general agreement with either the closed-loop (Hones, 1977) or very small pitch angle flux rope (Hughes and Sibeck, 1987; Birn et al., 1989) models of plasmoid structure and poorer agreement with other hypotheses. Calculations based upon typical plasmoid and tail parameters are presented, indicating that the J and B force associated with the disconnected lobe field lines may be sufficient to accelerate plasmoids up to the speeds observed by ISEE 3. Overall, the energy expended in accelerating the plasmoids down the tail appears comparable to that dissipated in the inner magnetosphere and ionosphere. The study produces strong evidence in favor of the plasmoid model of substorm tail dynamics.
    Schlagwort(e): GEOPHYSICS
    Materialart: Journal of Geophysical Research (ISSN 0148-0227); 94; 15153-15
    Format: text
    Standort Signatur Erwartet Verfügbarkeit
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  • 10
    facet.materialart.
    Unbekannt
    In:  Other Sources
    Publikationsdatum: 2019-07-12
    Beschreibung: The hypothesis that the IMF penetrates plasmoids causing them to be three- rather than two-dimensional is tested by comparing observations of By within plasmoids and related tail structures to upstream IMF By data. The magnetic topologies that result from the mergings of closed plasma sheet flux tubes and open tail lobe flux tubes at a near-earth neutral line, and merging near the tail flanks are described and studied. The particle signals and isotropic electron distributions are examined. It is observed that the IMF By penetrates plasmoids and that their structure is three-dimensional. In the three-dimensional model of plasmoids the reconnected plasma sheet field lines form a magnetic flux-ropelike structure. The three-dimensional model is utilized to analyze stagnant, slowly moving and earthward moving structures.
    Schlagwort(e): GEOPHYSICS
    Materialart: Geophysical Research Letters (ISSN 0094-8276); 14; 636-639
    Format: text
    Standort Signatur Erwartet Verfügbarkeit
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