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  • 1
    Electronic Resource
    Electronic Resource
    Comparative analysis of 18 sex pheromone plasmids fromEnterococcus faecalis: detection of a new insertion element on pPD1 and implications for the evolution of this plasmid family (1996)
    Springer
    Molecular genetics and genomics 252 (1996), S. 640-647 
    Details
    ISSN: 1617-4623
    Keywords: Sex pheromone plasmids ; Enterococcus faecalis ; Insertion element IS1062 ; Plasmid evolution
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A new IS element, IS1062, related to the enterococcal IS elements IS6770 and IS1252, was detected in the 3′-terminus of the surface exclusion gene,sep1, of sex pheromone plasmid pPD1 inEnterococcus faecalis. pPD1-bearing cells lack the surface exclusion function, probably as a consequence of this insertion. Analysis of pAD1 and pPD1 sequences (7.5 kb and 2.7 kb, respectively) downstream of their aggregation substance genes revealed no similarity in these DNA regions. Detailed DNA/DNA hybridization studies using DNA probes specific for various pAD1-encoded genes needed for plasmid transfer indicated that the sex pheromone plasmids have evolved by repeated recombination and insertion of diverse transposable elements which presumably account for recent acquisition of antibiotic resistances.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02173969
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Comparative analysis of 18 sex pheromone plasmids from Enterococcus faecalis: detection of a new insertion element on pPD1 and implications for the evolution of this plasmid family (1996)
    Springer
    Molecular genetics and genomics 252 (1996), S. 640-647 
    Details
    ISSN: 1617-4623
    Keywords: Key words Sex pheromone plasmids ; Enterococcus faecalis ; Insertion element IS1062 ; Plasmid evolution
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  A new IS element, IS1062, related to the enterococcal IS elements IS6770 and IS1252, was detected in the 3′-terminus of the surface exclusion gene, sep1, of sex pheromone plasmid pPD1 in Enterococcus faecalis. pPD1-bearing cells lack the surface exclusion function, probably as a consequence of this insertion. Analysis of pAD1 and pPD1 sequences (7.5 kb and 2.7 kb, respectively) downstream of their aggregation substance genes revealed no similarity in these DNA regions. Detailed DNA/DNA hybridization studies using DNA probes specific for various pAD1-encoded genes needed for plasmid transfer indicated that the sex pheromone plasmids have evolved by repeated recombination and insertion of diverse transposable elements which presumably account for recent acquisition of antibiotic resistances.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02173969
    Location Call Number Expected Availability
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    Paper (German National Licenses)
    Fulltext
  • 3
    Electronic Resource
    Electronic Resource
    Use of electroporation in genetic analysis of enterococcal virulence (1998)
    Springer
    Methods in cell science 20 (1998), S. 79-84 
    Details
    ISSN: 1573-0603
    Keywords: Aggregation substance ; Electroporation ; Enterococcal binding substance ; Enterococcus faecalis ; Virulence
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract We present two methods for electroporation for the gram positive bacterium Enterococcus faecalis that can also be used as guidelines for work with other gram positive species. We demonstrate the use and the advantages of this technique for investigating genes, both chromosomal and plasmid-linked, encoding surface structures. Electroporation was used to deliver constructs created on shuttle vectors for insertional inactivation of a chromosomal gene involved in binding substance formation as well as for the expression of Aggregation Substance in strains with different chromosomal backgrounds. The influence of defects in lipoteichoic acid synthesis and the expression of Aggregation Substance on virulence was shown in a rabbit endocarditis model.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1009890826383
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    Paper (German National Licenses)
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