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  • 1
    Electronic Resource
    Electronic Resource
    The Aspergillus nidulans CCAAT-binding factor AnCP/AnCF is a heteromeric protein analogous to the HAP complex of Saccharomyces cerevisiae (1998)
    Springer
    Molecular genetics and genomics 257 (1998), S. 404-411 
    Details
    ISSN: 1617-4623
    Keywords: Key wordsAspergillus ; CCAAT ; HapC ; Hap complex ; DNA binding
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The Aspergillus nidulans hapC gene was expressed as a fusion protein with MalE or glutathione-S-transferase (GST) in Escherichia coli, and used for the purification of HapC and the preparation of anti-HapC antiserum. The CCAAT-binding factor AnCP/AnCF contains a component with an approximate molecular mass of 32 kDa that cross-reacts with the antibody. The MalE-HapC fusion protein was able to replace authentic HapC in AnCP when incubated under appropriate conditions. Furthermore, reconstitution experiments with recombinant HapC, yHAP2 and yHAP5 polypeptides showed that all three polypeptides were required for the assembly of a complex capable of binding to CCAAT-containing taaG2 promoter DNA. The relationship between AnCP/AnCF and the Saccharomyces cerevisiae HAP complex is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004380050664
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  • 2
    Electronic Resource
    Electronic Resource
    ThehapC gene ofAspergillus nidulans is involved in the expression of CCAAT-containing promoters (1996)
    Springer
    Molecular genetics and genomics 251 (1996), S. 412-421 
    Details
    ISSN: 1617-4623
    Keywords: Aspergillus ; HAP3 ; CCAAT ; Gene regulation ; DNA binding
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The 5′ regulatory region of theamdS gene ofAspergillus nidulans, which encodes an acetamidase required for growth on acetamide as a carbon and nitrogen source, contains a CCAAT sequence which is required for setting the basal level ofamdS expression. Mobility shift studies have identified a factor inA. nidulans nuclear extracts which binds to this CCAAT sequence. InSaccharomyces cerevisiae theHAP3 gene encodes one component of a multisubunit complex that binds CCAAT sequences. A search of the EMBL and SwissProt databases has revealed anA. nidulans sequence with significant homology to theHAP3 gene adjacent to the previously cloned regulatory geneamdR. Sequencing of the remainder of this region has confirmed the presence of a gene, designatedhapC, with extensive homology toHAP3. The predicted amino acid sequence of HapC shows extensive identity to HAP3 in the central conserved domain, but shows little conservation in the flanking sequences. A haploid carrying ahapC deletion has been created and is viable, but grows poorly on all media tested. This null mutant grows especially slowly on acetamide as a sole carbon and nitrogen source, indicating thathapC plays a role inamdS expression. In agreement with this notion, it has been shown that thehapC deletion results in reduced levels of expression of anamdS::lacZ reporter gene and this effect is particularly evident under conditions of carbon limitation. Nuclear extracts prepared from thehapC deletion mutant show no CCAAT binding activity to theamdS orgatA promoters, indicating thathapC may encode a component of the complex binding at this sequence.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02172369
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  • 3
    Electronic Resource
    Electronic Resource
    On numerical modelling of embedded subsonic flow (1986)
    Chichester : Wiley-Blackwell
    International Journal for Numerical Methods in Fluids 6 (1986), S. 103-112 
    Details
    ISSN: 0271-2091
    Keywords: Supersonic ; Subsonic ; Mach Disc ; Compressible Pressure-correction ; Engineering ; Engineering General
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: An analytical model has been developed for computing embedded subsonic flow in rocket plumes from underexpanded axisymmetric supersonic nozzles. Numerical procedures based on the analysis have been incorporated in a simplified, non-reacting exhaust structure program and calculations for representative plume conditions performed. The technique is numerically stable and has provided satisfactory predictions of Mach-disc associated embedded subsonic flow.
    Additional Material: 7 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/fld.1650060302
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  • 4
    Electronic Resource
    Electronic Resource
    Iterative solution of the stream function-vorticity equations using a multigrid solver with finite elements (1993)
    Chichester : Wiley-Blackwell
    Communications in Numerical Methods in Engineering 9 (1993), S. 587-594 
    Details
    ISSN: 1069-8299
    Keywords: Engineering ; Engineering General
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Mathematics , Technology
    Notes: The solution of the stream function-vorticity equations is developed using a multigrid method to improve efficiency in a finite-element p-method. The equations are discretized using 2-D Lagrange finite elements (linear, quadratic and cubic) and solved in iteratively decoupled form using successive approximation and continuation methods. The comparative performance of several multigrid and direct matrix solvers is investigated with respect to convergence characteristics and time performance on the Cray Y-MP.
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/cnm.1640090706
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