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  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Protoplasma 196 (1997), S. 65-68 
    ISSN: 1615-6102
    Keywords: Nitrate uptake ; Triticum aestivum L. ; pH-stat ; Crop improvement ; Proton secretion
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Nitrate uptake and its link to net proton secretion in wheat (Triticum aestivum L. cv. Tassilo, Caribo, and Astron) were investigated using a pH-stat system. Since nitrate is taken up in symport with protons, nitrate and proton fluxes should be correlated. Nitrate concentration in the medium, measured by HPLC, decreased in a linear manner. The addition of nitrate caused a drop in net proton secretion rate to negative values (net proton influx). Once nitrate concentration had been lowered to a well defined level, net proton secretion rate started to recover. This critical nitrate concentration depended on the initial nitrate concentration in the medium. A technique to derive nitrate uptake rates from time courses of net proton secretion was developed and is described. Briefly, this method requires the initial nitrate concentration and the time until the minimal net proton secretion rate is achieved. Results determined with this technique were found in excellent agreement to simultaneous direct measurements of nitrate uptake by HPLC. Measurement of net proton secretion therefore can be used as a parameter for nitrate uptake and as a screening method for uptake efficiency. This method was used to compare three varieties of a high nitrogen efficiency breeding line of wheat. The originally less nitrogen efficient variety outperformed the actually sold cultivar in nitrate uptake rate.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Chichester [u.a.] : Wiley-Blackwell
    International Journal for Numerical Methods in Engineering 41 (1998), S. 721-738 
    ISSN: 0029-5981
    Keywords: surface crack ; stress intensity factor ; averaged error ; boundary-element method ; Engineering ; Numerical Methods and Modeling
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Mathematics , Technology
    Notes: The paper focuses on the comparison of different methods for calculating stress intensity factors (KI) in surface crack problems based on results of numerical analyses of elastic crack-tip fields. The computational accuracy is quantified by means of the so-called ‘averaged error estimation technique’ which is extended to the evaluation of local errors in the determination of stress intensity factors at characteristic points of the crack front. Numerical data involved in the present study are obtained from boundary-element calculations. Three values of the stress intensity factor, i.e. those defined from nodal tractions, displacements and energy-release rate, are provided. The highest error level is found for the displacement-based data, while the energy-release calculations yield the best accuracy. A considerable increase in the error value is noticed near the intersection of the crack front with a body surface where the conventional assumption on the square-root stress singularity is, in general, not applied. It is shown that the accuracy of stress intensity factor analysis can be improved by eliminating uncertainties associated with the local stress state along the crack front. © 1998 John Wiley Sons, Ltd.
    Additional Material: 10 Ill.
    Type of Medium: Electronic Resource
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