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  • 1
    Electronic Resource
    Electronic Resource
    Partial purification and characterization of a DNA helicase from chloroplasts of Glycine max (1990)
    Springer
    Plant molecular biology 15 (1990), S. 457-464 
    Details
    ISSN: 1573-5028
    Keywords: chloroplasts ; DNA helicase ; DNA replication
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A DNA helicase activity was detected in extracts of purified chloroplasts from the SB-1 cell line of Glycine max and partially purified by column chromatography on DEAE cellulose, phosphocellulose, and single-stranded DNA cellulose. The chloroplast helicase has a DNA-dependent ATPase activity, and its strand displacement activity is strictly dependent upon the presence of a nucleoside triphosphate and Mg2+ or Mn2+. Strand displacement activity does not require a free unannealed single-strand or replication fork-like structure.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00019162
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  • 2
    Electronic Resource
    Electronic Resource
    Analysis of soybean chloroplast DNA replication by two-dimensional gel electrophoresis (1993)
    Springer
    Plant molecular biology 23 (1993), S. 779-792 
    Details
    ISSN: 1573-5028
    Keywords: chloroplast ; DNA replication ; origin ; soybean ; two-dimensional gel electrophoresis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Chloroplast DNA replication was studied in the green, autotrophic suspension culture line SB-1 of Glycine max. Three regions (restriction fragments Sac I 14.5, Pvu II 4.1 and Pvu II 14.8) on the plastome were identified that displayed significantly higher template activity in in vitro DNA replication assays than all other cloned restriction fragments of the organelle genome, suggesting that these clones contain sequences that are able to direct initiation of DNA replication in vitro. In order to confirm that the potential in vitro origin sites are functional in vivo as well, replication intermediates were analyzed by two-dimensional gel electrophoresis using cloned restriction fragments as probes. The two Pvu II fragments that supported deoxynucleotide incorporation in vitro apparently do not contain a functional in vivo replication origin since replication intermediates from these areas of the plastome represent only fork structures. The Sac I 14.5 chloroplast DNA fragment, on the other hand, showed intermediates consistent with a replication bubble originating within its borders, which is indicative of an active in vivo origin. Closer examination of cloned Sac I 14.5 sub-fragments confirmed high template activity in vitro for two, S/B 5 and S/B 3, which also seem to contain origin sites utilized in vivo as determined by two-dimensional gel electrophoresis. The types of replication intermediate patterns obtained for these sub-fragments are consistent with the double D-loop model for chloroplast DNA replication with both origins being located in the large unique region of the plastome [17, 18]. This is the first report of a chloroplast DNA replication origin in higher plants that has been directly tested for in vivo function.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00021533
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  • 3
    Electronic Resource
    Electronic Resource
    A comparasion of three resequencing algorithms for the reduction of matrix profile and wavefront (1979)
    Chichester [u.a.] : Wiley-Blackwell
    International Journal for Numerical Methods in Engineering 14 (1979), S. 837-853 
    Details
    ISSN: 0029-5981
    Keywords: Engineering ; Engineering General
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Mathematics , Technology
    Notes: Three widely-used nodal resequencing algorithms were tested and compared for their ability to reduce matrix profile and root-mean-square (rms) wavefront, the latter being the most critical parameter in determining matrix decomposition time in the NASTRAN finite element computer program. The three algorithms are Cuthill-McKee (CM), Gibbs-Poole-Stockmeyer (GPS), and Levy. Results are presented for a diversified collection of 30 test problems ranging in size from 59 to 2680 nodes. It is concluded that GPS is exceptionally fast, and, for the conditions under which the test was made, the algorithm best able to reduce profile and rms wavefront consistently well. An extensive bibliography of resequencing algorithms is included.
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/nme.1620140606
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  • 4
    Electronic Resource
    Electronic Resource
    Structural analogies for scalar field problems (1981)
    Chichester [u.a.] : Wiley-Blackwell
    International Journal for Numerical Methods in Engineering 17 (1981), S. 471-476 
    Details
    ISSN: 0029-5981
    Keywords: Engineering ; Engineering General
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Mathematics , Technology
    Notes: This paper describes how general-purpose finite element structural analysis computer programs can be used, without modification, to solve various scalar field equations such as the wave equation, the Helmholtz equation, Laplace's equation, Poisson's equation, the heat equation and the telegraph equation, as well as mixed field problems (such as coupled structural-acoustic problems) which involve these equations.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/nme.1620170313
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