Publication Date:
2005-07-05
Description:
Technologies to assess the molecular targets of biomolecules in living cells are lacking. We have developed a technology called magnetism-based interaction capture (MAGIC) that identifies molecular targets on the basis of induced movement of superparamagnetic nanoparticles inside living cells. Efficient intracellular uptake of superparamagnetic nanoparticles (coated with a small molecule of interest) was mediated by a transducible fusogenic peptide. These nanoprobes captured the small molecule's labeled target protein and were translocated in a direction specified by the magnetic field. Use of MAGIC in genome-wide expression screening identified multiple protein targets of a drug. MAGIC was also used to monitor signal-dependent modification and multiple interactions of proteins.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Won, Jaejoon -- Kim, Mina -- Yi, Yong-Weon -- Kim, Young Ho -- Jung, Neoncheol -- Kim, Tae Kook -- New York, N.Y. -- Science. 2005 Jul 1;309(5731):121-5.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Biological Sciences, Korea Advanced Institute of Science and Technology, Daejeon 305-701, Korea.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/15994554" target="_blank"〉PubMed〈/a〉
Keywords:
Caspase 3
;
Caspases/metabolism
;
Cell Line
;
*Cell Physiological Phenomena
;
Cell Survival
;
Endocytosis
;
Fluorescein-5-isothiocyanate
;
Fluorescent Dyes
;
HeLa Cells
;
Humans
;
I-kappa B Proteins/metabolism
;
*Magnetics
;
Microscopy, Confocal
;
*Molecular Probe Techniques
;
*Molecular Probes
;
NF-kappa B/metabolism
;
*Nanostructures
;
Oligopeptides/metabolism
;
Phosphorylation
;
Protein Binding
;
Proteins/*metabolism
;
Quantum Dots
;
Recombinant Fusion Proteins/metabolism
;
Signal Transduction
;
Streptavidin
;
Tacrolimus/metabolism
;
Transcription Factor RelA
;
Tumor Necrosis Factor-alpha/pharmacology
;
beta-Transducin Repeat-Containing Proteins/metabolism
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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