ISSN:
1432-203X
Keywords:
Key wordsBeta vulgaris L.
;
Cryopreservation
;
Encapsulation-dehydration technique
;
Abscisic acid
;
Cold acclimation
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract It has been previously shown that shoot tips of in vitro plantlets of sugar beet (Beta vulgaris L. clone SES1) can be cryopreserved using the encapsulation-dehydration technique (survival rate of 37% after freezing). This article reports the influence of abscisic acid (ABA) and cold acclimation on survival after cryopreservation. When ABA was added to the multiplication medium of the plants, the survival rate of shoot tips after cryopreservation was not increased (45%). After cold acclimation of the plants, their growth pattern differed (plants became apically dominant) and the survival rate of the shoot tips after cryopreservation clearly increased (70% survival and 50% plant regeneration after freezing). This improved protocol was successfully applied to three other clones.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/s002990050484
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