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  • Elasmobranchii  (2)
  • 5′ nucleotidase  (1)
  • Springer  (3)
  • Chicago [u.a.] : The Univ. of Chicago Press
  • 1980-1984  (3)
  • 1920-1924
  • 1905-1909
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Verlag/Herausgeber
  • Springer  (3)
  • Chicago [u.a.] : The Univ. of Chicago Press
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Jahr
  • 1
    ISSN: 1432-1424
    Schlagwort(e): plasma membrane ; lung ; histamine receptors ; 5′ nucleotidase
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Chemie und Pharmazie
    Notizen: Summary A simple and rapid method of isolating plasma membranes from human peripheral lung tissue is described. The method involves homogenization of tissue in 0.25m sucrose-buffered medium followed by differential and sucrose density gradient centrifugation. Enzymatic and morphological characterization of the plasma membrane fraction revealed minimal contamination by nonplasma membrane fragments. The isolated plasma membranes showed an 18-fold purification of 5′-nucleotidase activity compared to the original homogenate. Electronmicroscopic studies of the plasma membrane fraction revealed the presence of small membrane vesicles having a trilaminar membrane structure. To further examine the purity of the plasma membrane preparation, the binding of the H1 receptor antagonist,3H pyrilamine, to the plasma membrane-enriched fraction was compared to the binding to crude membrane preparations. Both the plasma membrane-enriched fraction and the crude membrane preparation had similar Kd's for the histamine antagonist, but the plasma membrane-enriched fraction had a threefold greater binding capacity, reflecting the relative enrichment of plasma membranes of the preparation. Thus, a method has been developed for the isolation of plasma membranes from human peripheral lung which should provide material for a variety of biochemical and pharmacological studies.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 2
    Digitale Medien
    Digitale Medien
    Springer
    Environmental biology of fishes 9 (1983), S. 3-24 
    ISSN: 1573-5133
    Schlagwort(e): Amazonia ; Batoidea ; Brazil ; Captive breeding ; Chondrichthyes ; Colombia ; Elasmobranchii ; Freshwater adaptation ; Growth rate ; Potamotrygonidae
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Synopsis Observations of reproductive features and body measurements were made on wild-caught, freshwater stingrays, Potamotrygon circularis and P. motoro, from the Amazon drainage of western Brazil and southern Colombia. Further observations were made in Detroit's Belle Isle Aquarium on a captive pair of P. motoro and their descendants, which constitute the first known captive breeding colony of potamotrygonids. The gross structure and function of female and male reproductive systems are described. There is no obvious difference between those of the two species. They are aplacentally viviparous, the young being nourished in advanced stages by uterine milk secreted by trophonemata. Size at onset and completion of sexual maturation, breeding season and behavior, gestation period, litter size and sex ratios are discussed. Up to 21 proportional measurements were made on several fetal and postnatal stages of both species. Several proportional changes occur in very early fetal life, but most body proportions undergo only minor changes from advanced fetal through adult stages. A growth curve is proposed for P. motoro based on observations of the captive colony.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 3
    ISSN: 1573-5133
    Schlagwort(e): Elasmobranchii ; Euryhalinity ; Freshwater adaptation ; Dam lake ; Osmoregulation ; Central America
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Synopsis The Río Bayano in eastern Panama is one of many tropical rivers in which bull sharks (Carcharhinus leucas) and largetooth sawfish (Pristis perotteti) have been known to occur. Since both species can osmoregulate in fresh water throughout life, theoretically, both could survive in landlocked situations for many years.P. perotteti reproduces in fresh water, butC. leucas ordinarily does not, so only the former would appear to have the potential for establishing a breeding stock in such a landlocked freshwater body. The damming of the Rio Bayano and the creation of a large impoundment in 1976 provides a test of the ability of members of both species trapped there to survive and to establish breeding stocks. In 1980 and 1981 three mature femaleC. leucas were found dead in Lake Bayano and three sub-adult femaleP. perotteti were taken by trammel net. These events confirm the ability of both to survive in fresh water for long periods, but the establishment of breeding stocks appears doubtful and the question may not be answered for many years.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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