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  • Earthworm, Allolobophora caliginosa  (1)
  • Karyometry  (1)
  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Cell & tissue research 151 (1974), S. 171-186 
    ISSN: 1432-0878
    Keywords: Cerebral ganglion ; Earthworm, Allolobophora caliginosa ; Regeneration ; Monoaminergic neurons ; Microspectrofluorimetry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The regeneration of monoaminergic neurons in the cerebral (supraesophageal) ganglion of the earthworm, Allolobophora caliginosa, was studied by the fluorescence method of Falck-Hillarp 3, 4, 5, and 6 weeks after extirpation of the ganglion. The monoamine fluorophores in control and regenerating cerebral ganglia were estimated by microspectrofluorimetric analysis. In the control animals catecholaminergic (noradrenergic, dopaminergic) and indolaminergic (5-HT/5-HTP) neurons were observed. Four weeks after the removal of the cerebral ganglia, two types of monoamine-containing neurons reappeared. Microspectrofluorimetrically dopaminergic and indolaminergic perikarya were identified. Reconstructions from serial sections and counts of the regenerated fluorescent cells showed no further increase in the number of these cells between the 5th and 6th postoperative weeks. In the 5- and 6-week groups the intrinsic and afferent aminergic pathways of the cerebral ganglion were reestablished.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Cell & tissue research 143 (1973), S. 143-147 
    ISSN: 1432-0878
    Keywords: Rat ; Suprachiasmatic nucleus ; Blinding ; Retinohypothalamic connections ; Karyometry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Zusammenfassung Radioaktiv markierte Aminosäuren werden bei Säugetieren nach intraokulärer Injektion nicht nur zu den primären optischen Kerngebieten, sondern auch in den Nucleus suprachiasmaticus des Hypothalamus transportiert (Moore und Lenn, 1972; Hendrickson, Wagoner und Cowan, 1972; Moore, 1973). Dieser Befund macht eine direkte Verknüpfung zwischen der Netzhaut und dem sekretorisch aktiven Nucleus suprachiasmaticus wahrscheinlich, obwohl mit Silbertechniken eine solche Verbindung nicht sicher nachgewiesen werden konnte. Karyometrische Studien am Nucleus suprachiasmaticus männlicher Wistar-Ratten zeigen 6 Tage nach beidseitiger Blendung eine signifikante Abnahme der Zellkerndurchmesser. Dieses Ergebnis spricht für eine Aktivitätsänderung der Neurone im Nucleus suprachiasmaticus geblendeter Ratten.
    Notes: Summary Intraocular injection of labeled amino acids results, in different mammals, in an accumulation of radioactive material not only in the primary optic centers but also within the hypothalamic suprachiasmatic nucleus (Moore et al., 1972; Hendrickson et al., 1972; Moore, 1973). This finding argues in favour of a direct connection between the retina and the secretory suprachiasmatic nucleus although silver techniques do not show such a pathway. In male Wistar rats the nuclear diameter of the suprachiasmatic neurons decreases significantly 6 days after bilateral experimental ablation of the retina. These results indicate an alteration in activity of the suprachiasmatic neurons in blinded rats.
    Type of Medium: Electronic Resource
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