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  • 1
    Electronic Resource
    Electronic Resource
    New disease symptoms observed on field-grown potato plants with potato spindle tuber viroid and potato virus Y infections (1987)
    Springer
    Potato research 30 (1987), S. 127-133 
    Details
    ISSN: 1871-4528
    Keywords: Synergistic reaction ; necrotic symptoms ; virus concentration ; ELISA ; PAGE
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Potato spindle tuber viroid (PSTV) and potato virus Y (PVY) were isolated from plants of cultivar Kennebec with severe necrotic symptoms in the field. In the greenhouse, severe necrotic symptoms were reproduced only when potato plants were infected either simultaneously with PSTV+PVY, or with PSTV prior to PVY infection. Thirteen additional potato cultivars were tested in the greenhouse for this synergistic reaction and eight developed necrotic responses similar to cv. Kennebec. PVY concentration was significantly higher in doubly infected plants, compared with those infected with PVY alone.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02357692
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Biotinylated DNA probes for detecting virus Y and aucuba mosaic virus in leaves and dormant tubers of potato (1992)
    Springer
    Potato research 35 (1992), S. 173-182 
    Details
    ISSN: 1871-4528
    Keywords: non-radioactive probes ; RNA or DNA hybridization ; ELISA ; potato virus Y ; potato aucuba mosaic virus
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Detection of potato virus Y (PVY) in dormant potato tubers using the enzyme-linked immunosorbent assay (ELISA) has been reported not to be accurate and reliable and it requires breaking of the dormancy of tubers prior to testing. We describe a simple, practical and highly sensitive hybridization method based on the use of biotinylated DNA probes for detecting PVY and potato aucuba mosaic virus (PAMV) in dilutions made of crude extracts of infected potato leaves and dormant tubers. As little as 50 fg of RNA can be detected by this method, and the probes are highly specific for their targets, even in crude plant extracts. The presence of one virus did not interfere with the detection of the other.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02357611
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    Paper (German National Licenses)
    Fulltext
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