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  • 1
    Electronic Resource
    Electronic Resource
    The Aspergillus nidulans CCAAT-binding factor AnCP/AnCF is a heteromeric protein analogous to the HAP complex of Saccharomyces cerevisiae (1998)
    Springer
    Molecular genetics and genomics 257 (1998), S. 404-411 
    Details
    ISSN: 1617-4623
    Keywords: Key wordsAspergillus ; CCAAT ; HapC ; Hap complex ; DNA binding
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The Aspergillus nidulans hapC gene was expressed as a fusion protein with MalE or glutathione-S-transferase (GST) in Escherichia coli, and used for the purification of HapC and the preparation of anti-HapC antiserum. The CCAAT-binding factor AnCP/AnCF contains a component with an approximate molecular mass of 32 kDa that cross-reacts with the antibody. The MalE-HapC fusion protein was able to replace authentic HapC in AnCP when incubated under appropriate conditions. Furthermore, reconstitution experiments with recombinant HapC, yHAP2 and yHAP5 polypeptides showed that all three polypeptides were required for the assembly of a complex capable of binding to CCAAT-containing taaG2 promoter DNA. The relationship between AnCP/AnCF and the Saccharomyces cerevisiae HAP complex is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004380050664
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    ThehapC gene ofAspergillus nidulans is involved in the expression of CCAAT-containing promoters (1996)
    Springer
    Molecular genetics and genomics 251 (1996), S. 412-421 
    Details
    ISSN: 1617-4623
    Keywords: Aspergillus ; HAP3 ; CCAAT ; Gene regulation ; DNA binding
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The 5′ regulatory region of theamdS gene ofAspergillus nidulans, which encodes an acetamidase required for growth on acetamide as a carbon and nitrogen source, contains a CCAAT sequence which is required for setting the basal level ofamdS expression. Mobility shift studies have identified a factor inA. nidulans nuclear extracts which binds to this CCAAT sequence. InSaccharomyces cerevisiae theHAP3 gene encodes one component of a multisubunit complex that binds CCAAT sequences. A search of the EMBL and SwissProt databases has revealed anA. nidulans sequence with significant homology to theHAP3 gene adjacent to the previously cloned regulatory geneamdR. Sequencing of the remainder of this region has confirmed the presence of a gene, designatedhapC, with extensive homology toHAP3. The predicted amino acid sequence of HapC shows extensive identity to HAP3 in the central conserved domain, but shows little conservation in the flanking sequences. A haploid carrying ahapC deletion has been created and is viable, but grows poorly on all media tested. This null mutant grows especially slowly on acetamide as a sole carbon and nitrogen source, indicating thathapC plays a role inamdS expression. In agreement with this notion, it has been shown that thehapC deletion results in reduced levels of expression of anamdS::lacZ reporter gene and this effect is particularly evident under conditions of carbon limitation. Nuclear extracts prepared from thehapC deletion mutant show no CCAAT binding activity to theamdS orgatA promoters, indicating thathapC may encode a component of the complex binding at this sequence.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02172369
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  • 3
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    The use of Skylab data to study the early detection of insect infestations and density and distribution of host plants (1975)
    In:  CASI
    Details
    Publication Date: 2019-06-27
    Description: The detection of insect infestations and the density and distribution of host plants were studied using Skylab data, aerial photography and ground truth simultaneously. Additional ground truth and aerial photography were acquired between Skylab passes. Three test areas were selected: area 1, of high density citrus, was located northwest of Mission, Texas; area 2, 20 miles north of Weslaco, Texas, irrigated pastures and brush-covered land; area 3 covered the entire Lower Rio Grande Valley and adjacent areas of Mexico. A color composite picture of S-190A data showed patterns of vegetation on both sides of the Rio Grande River clearly delineating the possible avenues of entry of pest insects from Mexico into the United States or from the United States into Mexico. Vegetation that could be identified with conventional color and color IR film included: citrus, brush, sugarcane, alfalfa, irrigated and unimproved pastures.
    Keywords: EARTH RESOURCES AND REMOTE SENSING
    Type: A-15 , NASA. Lyndon B. Johnson Space Center. NASA Earth Resources Survey Symp. Vol. 1-A: Agr. Environment; p 203-219
    Format: application/pdf
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  • 4
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    Unknown
    A study of the early detection of insect infestations and density/distribution of host plants (1975)
    In:  CASI
    Details
    Publication Date: 2019-06-27
    Description: The author has identified the following significant results. With comparative observations of film types and seasonal influences on reflectance characteristics, many crop varieties can be identified. This study shows that citrus, sugar cane, brush, some winter vegetables, and grain crops could be identified. Vegetative patterns in border areas can be detected. This information can be useful in detecting avenues of entry of pest species and areas of stress that require vigilance in stopping the spread of destructive species. Influence of some environmental factors on crops that may be confused with pest injury, or related factors, can be detected and identified with Skylab data (S-190B).
    Keywords: EARTH RESOURCES AND REMOTE SENSING
    Type: E76-10079 , NASA-CR-144483
    Format: application/pdf
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  • 5
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    Unknown
    A study of the early detection of insect infestations and density/distribution of host plants (1974)
    In:  CASI
    Details
    Publication Date: 2019-07-13
    Description: There are no author-identified significant results in this report.
    Keywords: EARTH RESOURCES AND REMOTE SENSING
    Type: E75-10116 , NASA-CR-141951 , PR-18
    Format: application/pdf
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  • 6
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    Unknown
    A study of the early detection of insect infestations and density/distribution of host plants (1974)
    In:  CASI
    Details
    Publication Date: 2019-07-13
    Description: There are no author-identified significant results in this report.
    Keywords: EARTH RESOURCES AND REMOTE SENSING
    Type: E75-10115 , NASA-CR-141950 , PR-19
    Format: application/pdf
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  • 7
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    Unknown
    A study of the early detection of insect infestations and density/distribution of host plants (1974)
    In:  CASI
    Details
    Publication Date: 2019-07-13
    Description: The author has identified the following significant results. Significant results have been obtained in the identification of citrus, sugarcane, winter vegetables, irrigated pastures, and unimproved pastures which contain brush. Land without vegetation, lakes, roads, and waterways can also be determined. Different densities of vegetation covering some cultivated areas are apparent. The practical applications of these results are many. The abundance of host plants of pests can be determined. Avenues of entry of pests can be plotted, facilitating control or preventing entry of pest species. The boundaries of areas to be quarantined can be accurately established after viewing the S-190B data. Better cultural methods can be employed such as planning where to plant certain crops that indirectly are detrimental to those already growing. This would relate to such factors as pesticide drift or alternate hosts of major pests.
    Keywords: EARTH RESOURCES AND REMOTE SENSING
    Type: E75-10062 , NASA-CR-141002 , PR-17
    Format: application/pdf
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