ISSN:
0935-6304
Keywords:
Dry-packing in liquid chromatography
;
Lateral tapping beneficial
;
Vertical tapping detrimental
;
Manual versus automatic packing
;
Chemistry
;
Analytical Chemistry and Spectroscopy
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Chemistry and Pharmacology
Notes:
This contribution comprises two parts, the one covering some variables in the dry-packing of columns for liquid chromatography, the other the use of an automatic dry-packer in liquid chromatography. A high-pressure stainless steel column was dry-packed using a 20-44 μm size, irrregularly shaped, silica gel support. It was found that, when support was added in discrete amounts, lateral tapping produced a column of higher efficiency (HETP = 0.69 mm) than was the case for vertical tapping (HETP = 1.37 mm). Simultaneous vertical and lateral tapping produced an intermediate result. Bulk filling of the column followed by tapping was inferior to adding support in discrete amounts. An apparatus constructed to perform the vertical and lateral tapping operations automatically confirmed the above conclusions. It was concluded that commercially available dry-packing apparatus do not offer the best action for packing an efficient column since they involve predominantly vertical tapping.
Additional Material:
2 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/jhrc.1240040605
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