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  • Drosophila melanogaster  (4)
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  • 1
    ISSN: 1573-6857
    Keywords: Drosophila melanogaster ; isofemale lines ; isogroups ; natural populations ; pigmentation ; body size
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Studies of short or medium range geographic variations play an increasing role in ecological genetics, and sensitive techniques are required to detect them. In this respect, two sampling techniques were compared inD. melanogaster. The biological data were provided by the analysis of four natural populations from the same geographic area, Spain (one) and Southern France (three), for four morphometrical traits: abdomen and thoracic pigmentation, and wing and thorax lengths. Traits were measured on wild living females and on their progeny reared in the laboratory at 25°C. For progeny analyses, two techniques were compared: the usual isofemale line technique, sib families issued from a single female, and a new isogroup technique, the progeny produced by a group of 20 wild-collected parents. Large phenotypic variations were observed in wild living flies, corresponding to the unstability of natural environmental conditions during their development. Among laboratory grown flies, variations were much smaller. Between isogroups, differences were small, due to sampling error and some common environment effects. Variations between lines were much greater, thus demonstrating a strong genetic component. When different populations have to be compared, the isogroup technique should be preferred since, for the same amount of work, the lesser variability between groups provides a more precise characterization of the population means.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Cellular and molecular life sciences 51 (1995), S. 744-748 
    ISSN: 1420-9071
    Keywords: Morphometry ; geographic races ; chromosome transfers ; Drosophila melanogaster ; natural populations
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract The genetic basis of three morphological traits (ovariole number, sternopleural bristle number and wing length) ofDrosophila melanogaster has been investigated in natural populations that show great differences in these traits, i. e. Bordeaux (France) and Loua (Congo). F1 and F2 crosses, and chromosome substitutions between these two populations, were analysed. Maternal and/or X chromosome effects were found for sternopleural bristle number and wing length. For all traits, significant effects from each of the three chromosomes were found, but in general only one or two chromosomes had a major effect. Moreover, in all cases significant interactions between chromosomes were observed, suggesting the existence of epistatic effects. Our results are discussed and compared to those obtained from the analysis of selected laboratory strains.
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 1573-3297
    Keywords: Drosophila melanogaster ; digging behavior ; larval depth concealment ; parasitic success
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Psychology
    Notes: Abstract TwoDrosophila strains were compared with respect to the behavior of their larvae on food substrate: a wild-type strain (D) from the West Indies exhibited digging behavior, while a laboratory strain (S), bearing theebony mutation, remained on the surface. Chromosome transfers showed this difference to be due mainly to autosomes. There was a significant difference between the two strains in the proportions of larvae parasitized by a cynipid wasp. This was not due to theebony mutation or to other traits such as larval size, cuticle thickness, and speed of development. Chromosome transfers demonstrated a significant role of the three major chromosomes in the susceptibility to the parasite. A clear parallelism was found between the susceptibility to parasitization and the proportion of surface larvae. The depth of concealment of the larvae in the food matter appears to be a favorable behavioral strategy for escaping parasite attacks. The possible adaptive significance of genetic variations in larval behavior is discussed.
    Type of Medium: Electronic Resource
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  • 4
    ISSN: 1573-4927
    Keywords: population ; Drosophila melanogaster ; D. simulans ; electrophoresis ; heterozygosity, allozymes ; genetic strategies ; adaptation ; bottleneck effect
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract An electrophoretic study was carried out to compare the geographic pattern of genetic variation in Drosophila simulans with that of its sibling species, Drosophila melanogaster. An identical set of 32 gene-protein loci was studied in four geographically distant populations of D. simulans and two populations of D. melanogaster, all originating from Europe and Africa. The comparison yielded the following results: (1) tropical populations of D. simulans were, in terms of the number of unique alleles, average heterozygosity per locus, and percentage of loci polymorphic, more variable than conspecific-temperate populations; (2) some loci in both species showed interpopulation differences in allele frequencies that suggest latitudinal clines; and (3) temperate-tropical genetic differentiation between populations was much less in D. simulans than in D. melanogaster. Similar differences between these two species have previously been shown for chromosomal, quantitative, physiological, and middle-repetitive DNA variation. Estimates of N m (number of migrants per generation) from the spatial distribution of rare alleles suggest that both species have similar levels of interpopulation gene flow. These observations lead us to propose two competing hypotheses: the low level of geographic differentiation in D. simulans is due to its evolutionarily recent worldwide colonization and, alternatively, D. simulans has a narrower niche than D. melanogaster. Geographic variation data on different genetic elements (e.g., mitochondrial DNA, two-dimensional proteins, etc.) are required before these hypotheses can be adequately tested.
    Type of Medium: Electronic Resource
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