ISSN:
0173-0835
Keywords:
Isotachophoresis
;
Displacement electrophoresis
;
Moving boundary
;
Tapered capillary
;
Nonuniform cross section
;
Chemistry
;
Biochemistry and Biotechnology
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Biology
,
Chemistry and Pharmacology
Notes:
The principle and theoretical description of boundary focusing under electrophoretic migration in a tapered capillary are presented. The model describes the gradual volume decrease of a moving boundary between weak electrolytes or ampholytes. The relations between the departure from the local steady state, the ratio of inlet to outlet capillary cross section, the effective mobilities and the selectivity are formulated. They allow the selection of operating conditions such that the departure from the local steady state can be decreased to an acceptable level. The relations predict that isotachophoresis (displacement electrophoresis) in a capillary with a shallow taper can maintain a constant ratio of actual boundary width to local steady state width, provided that the local capillary cross section decreases linearly with length. The significance of some assumptions needed for the model formulation is analyzed. Numerical examples are given indicating the method feasibility.
Additional Material:
5 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/elps.11501601335
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