ISSN:
1612-1112
Keywords:
Column liquid chromatography
;
Gas chromatography/mass spectrometry
;
Ligand exchange
;
Dipeptides
Source:
Springer Online Journal Archives 1860-2000
Topics:
Chemistry and Pharmacology
Notes:
Summary A two step process is described for the separation and identification of trace amounts of dipeptides in an hydrolysed brain extract. Peptides were first separated from the amino-acids by ligand exchange chromatography (LEC) on an iminodiacetate bonded polymer in the Cu(II) form. After elution from the LEC column, peptides were transformed into volatile derivatives and separated by gas chromatography (GC). This method presented many advantages in comparison with the liquid chromatography (LC) commonly used for peptide separations: a better efficiency due to the utilization of a capillary column, a better selectivity between the amino-acids and peptides and less interference from non-peptidic substances. Combination of GC with mass spectrometry allowed the sequence determination of twelve dipeptides in the extract. Three of them were quantitatively determined.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF02262244
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