Publication Date:
2004-08-21
Description:
Cartilaginous fish are the phylogenetically oldest living organisms known to possess components of the vertebrate adaptive immune system. Key to their immune response are heavy-chain, homodimeric immunoglobulins called new antigen receptors (IgNARs), in which the variable (V) domains recognize antigens with only a single immunoglobulin domain, akin to camelid heavy-chain V domains. The 1.45 angstrom resolution crystal structure of the type I IgNAR V domain in complex with hen egg-white lysozyme (HEL) reveals a minimal antigen-binding domain that contains only two of the three conventional complementarity-determining regions but still binds HEL with nanomolar affinity by means of a binding interface comparable in size to conventional antibodies.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Stanfield, Robyn L -- Dooley, Helen -- Flajnik, Martin F -- Wilson, Ian A -- GM38273/GM/NIGMS NIH HHS/ -- RR06603/RR/NCRR NIH HHS/ -- New York, N.Y. -- Science. 2004 Sep 17;305(5691):1770-3. Epub 2004 Aug 19.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Molecular Biology, Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/15319492" target="_blank"〉PubMed〈/a〉
Keywords:
Animals
;
Complementarity Determining Regions/chemistry
;
Crystallography, X-Ray
;
Dimerization
;
Drug Combinations
;
Evolution, Molecular
;
Genes, Immunoglobulin
;
Immunoglobulin Heavy Chains/*chemistry/genetics/metabolism
;
Immunoglobulin Variable Region/*chemistry/genetics/immunology/metabolism
;
Immunoglobulins/*chemistry/genetics/immunology/metabolism
;
Meglumine
;
Models, Molecular
;
Muramidase/*chemistry/immunology/metabolism
;
Protein Conformation
;
Protein Folding
;
Protein Structure, Tertiary
;
Receptors, Antigen/*chemistry/genetics/immunology/metabolism
;
Sharks/*immunology
;
Tetrahydropapaveroline/*analogs & derivatives
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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