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  • Bowman-Birk inhibitor  (1)
  • Dialysis  (1)
  • 1
    Electronic Resource
    Electronic Resource
    Thermodynamics of the Binding of Chymotrypsin with the Black-eyed Pea Trypsin and Chymotrypsin Inhibitor (BTCI) (1999)
    Springer
    The protein journal 18 (1999), S. 307-313 
    Details
    ISSN: 1573-4943
    Keywords: Bowman-Birk inhibitor ; microcalorimetry ; enthalpy ; fluorescence polarization
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract The binding of α-chymotrypsin to black-eyed pea trypsin/chymotrypsin inhibitor (BTCI) has been studied using the inhibitory activity against the enzyme and the formation of the complex enzyme/inhibitor followed by measurements of fluorescence polarization. Apparent equilibrium constants were estimated for several temperatures and the values obtained range from 0.32 × 107 to 1.36 × 107 M−1. The following values were found from van't Hoff plots: Δ H vh ° = 10.8 kcal mol-1 (from inhibitory assays) and 11.1 kcal mol−1 (from fluorescence polarization); ΔS° = 67.9 and = 67.8 kcal K−1 mol−1, respectively. Calorimetric binding enthalpy was determined (corrected for the ionization heat of the buffer) and the resulting value was ΔH cal ° = 4.9 kcal mol-1. These results indicate that the binding of chymotrypsin to BTCI is an entropically driven process.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1021039429014
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  • 2
    Electronic Resource
    Electronic Resource
    Programmable machine for dialyser reuse (1980)
    Springer
    Medical & biological engineering & computing 18 (1980), S. 765-771 
    Details
    ISSN: 1741-0444
    Keywords: Dialysis ; Dialyser reuse
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: Abstract Because of the high cost of dialysis, many centres have developed methods of cleaning diaylser cartridges so that they may be reused. This paper describes a machine which cleans dialyser cartridges automatically. The main difference between this machine and others reported in the literature is its ability to test automatically for leaks or obstruction and sound an alarm if the dialyser is unsuitable for reuse after the washing process. The development of these tests is decribed in detail. The incorporation of these tests into the alarm system of the machine will make the reuse of dialysers simpler, safer and more consistent.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02441903
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    Paper (German National Licenses)
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