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  • 1
    Electronic Resource
    Electronic Resource
    The effect of deuterium oxide on protein turnover in Lemna minor (1979)
    Springer
    Planta 146 (1979), S. 229-236 
    Details
    ISSN: 1432-2048
    Keywords: Density labelling ; Deuterium oxide ; Lemna ; Protein turnover
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Lemna minor fronds transferred to a sterile culture medium containing 50% (v/v) deuterium oxide (2H2O) rapidly undergo a loss of soluble protein with a corresponding increase in free amino acids. The loss of protein is due to two factors: (i) the inhibition of protein synthesis for 4 h followed by a slower rate of synthesis than normal, (ii) a rapid 9–10 fold increase in protein degradation. In plants grown for longer periods (3–6 days) in 50% 2H2O medium, protein synthesis is inhibited by 20% and the rate constant of degradation is 2–3 times that measured in fronds growing in normal (H2O containing) complete medium. The initial loss of protein is not due to the breakdown of any specific protein fraction. Investigation of several enzymes indicates that all proteins are catabolised in response to 2H2O treatment. The implications of these results with regard to the interpretation of density-labelling experiments are discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00388237
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  • 2
    Electronic Resource
    Electronic Resource
    The mechanism of deuterium oxide-induced protein degradation in Lemna minor (1980)
    Springer
    Planta 148 (1980), S. 374-380 
    Details
    ISSN: 1432-2048
    Keywords: Deuterium oxide ; Lemna ; Protein degradation ; Stress (D2O) ; Tonoplast properties
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Transfer of Lemna minor fronds to culture medium containing 50% (v/v) deuterium oxide induces a large increase in the rate of protein breakdown, which is not due to an increase in the activity of acidic or neutral proteolytic enzymes or peptidases. Biochemical and ultrastructural evidence indicates that deuterium oxide affects the properties of certain membranes, particularly the tonoplast, and allows vacuolar proteolytic enzymes to pass into the cytoplasm and cause the increased protein breakdown.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00388126
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    Paper (German National Licenses)
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