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  • 1
    Electronic Resource
    Electronic Resource
    Genetic transformation of Chrysanthemum using wild type Agrobacterium strains; strain and cultivar specificity (1991)
    Springer
    Plant cell reports 9 (1991), S. 505-508 
    Details
    ISSN: 1432-203X
    Keywords: Agrobacterium ; Tumour induction ; Dendranthema grandiflora ; Polymerase chain reaction ; ß-Glucuronidase ; Neomycine phosphotransferase
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary To develop an Agrobacterium mediated transformation protocol for chrysanthemum we studied the transformation efficiency of commonly used A.tumefaciens strains on 14 genotypes by comparing tumour size and frequency. One genotype was analyzed in detail using 14 strains of both A.tumefaciens and A.rhizogenes. Only a few genotype/strain combinations resulted in significant tumour formation. Especially 0-type strains were highly efficient. An 0-type strain was used to transfer genes for neomycine phosphotransferase (NPT II) and ß-glucuronidase (GUS) to a susceptible cultivar. Transfer of the GUS gene was confirmed by using the Polymerase Chain Reaction (PCR).
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00232106
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  • 2
    Electronic Resource
    Electronic Resource
    Life history studies of the leafminer Liriomyza trifolii on susceptible and resistant cultivars of Dendranthema grandiflora (1991)
    Springer
    Euphytica 56 (1991), S. 47-53 
    Details
    ISSN: 1573-5060
    Keywords: Chrysanthemum morifolium ; Dendranthema grandiflora ; host plant resistance ; leafminer ; life history ; Liriomyza trifolii
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The feeding, fecundity, mortality and rate of development of Liriomyza trifolii (Burgess) on 12 cultivars of Dendranthema grandiflora (Tzvel.) (Syn. Chrysanthemum morifolium (Ramat.)) were determined in ‘no choice’ experiments. Number of feeding punctures, number of eggs laid and total number of larvae varied between cultivars but these characters were not strongly associated with leafminer population increase. Good indicators of host plant resistance were larval mortality, number of pupae and duration of the larval period. Of these three, larval mortality which could be as high as 98% was most suitable. Based on life history data it was predicted that Liriomyza trifolii cannot survive on the resistant cultivar Penny Lane. This prediction was verified experimentally.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00041743
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  • 3
    Electronic Resource
    Electronic Resource
    Restoring adventitious shoot formation on chrysanthemum leaf explants following cocultivation with Agrobacterium tumefaciens (1993)
    Springer
    Plant cell, tissue and organ culture 32 (1993), S. 263-270 
    Details
    ISSN: 1573-5044
    Keywords: Dendranthema grandiflora ; preculture ; regeneration ; transformation ; β-glucuronidase
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Explants from leaves of in vitro-grown chrysanthemum (Dendranthema grandiflora Tzvel.) cultivars regenerated adventitious shoots without an intermediate callus phase. Puncturing explants with a brush increased regenerations, but in combination with cocultivation with Agrobacterium tumefaciens it had an adverse effect on shoot formation. The negative effect of brushing and cocultivation could be overcome by preculturing explants for 8 days. Preculture altered the location of transformed sites but did not inhibit transformation. Regeneration following cocultivation with Agrobacterium is also encouraged if alternative regeneration protocols are used that do not require brushing.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00042287
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