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  • 1
    Electronic Resource
    Electronic Resource
    Chimpanzee microsatellite PCR primers applied to paternity testing in a captive colony (1993)
    Springer
    Primates 34 (1993), S. 357-363 
    Details
    ISSN: 0032-8332
    Keywords: Pan troglodytes ; DNA typing ; Chimpanzee ; Paternity test ; Dinucleotide repeats ; GT/AC repeats
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Previously designed primers for the polymerase chain reaction (PCR) amplifying microsatellite DNA segments containing GT/AC dinucleotide repeats in the chimpanzee (Pan troglodytes) genome were used for paternity testing in a breeding colony in captivity. Combinations of three PCR primers identified the fathers of all the tested 40 chimpanzees born in an eight-year period. The results suggested: (1) a positive (though not conclusive) correlation between male rank and number of offspring; (2) choice of mating partners by the female rather than by the male; and (3) absence of stable mating pairs over the years. For studies of chimpanzees in captivity and in the wild, these primers should be useful for paternity testing, for investigating genetic variations, and for improving genetic maintenance of breeding colonies.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02382631
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  • 2
    Electronic Resource
    Electronic Resource
    Polymorphic microsatellite DNA amplification customized for chimpanzee paternity testing (1993)
    Springer
    Primates 34 (1993), S. 27-35 
    Details
    ISSN: 0032-8332
    Keywords: Pan troglodytes ; DNA typing ; Chimpanzee ; Paternity test ; Dinucleotide repeats ; GT/AC repeats
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract DNA segments containing GT/AC dinucleotide repeats in the chimpanzee (Pan troglodytes) genome were screened. Thirteen transformedE. coli colonies were identified with the (GT)10 probe to have chimpanzee DNA fragments containing (GT)n repeats. These potentially polymorphic (variable n) DNA segments were sequenced. Primers for the polymerase chain reaction (PCR) amplifying these DNA segments were designed. Six pairs of primers yielded polymorphic PCR products. Three of them revealed considerable length polymorphisms and heterozygosities in a group of captive chimpanzees. For studies on chimpanzees in the wild and in captivity, these primers should be useful for paternity testing, for investigating genetic variations, and for improving the genetic maintenance of breeding colonies. The strategy adopted in the present study to obtain PCR primers amplifying polymorphic microsatellite DNA segments may well be applicable to almost all eukaryotic organisms.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02381277
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  • 3
    Electronic Resource
    Electronic Resource
    Paternity discrimination and inter-group relationships of chimpanzees at Bossou (1993)
    Springer
    Primates 34 (1993), S. 545-552 
    Details
    ISSN: 0032-8332
    Keywords: Bossou (Guinea) ; Paternity: Inter-group relationship ; Chimpanzee ; DNA typing ; Migration
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A small group of wild chimpanzees at Bossou, southeastern Guinea, is semi-isolated, occupying a home-range which is several kilometers from those of other groups. The group has had only one adult male since 1985, raising the probability of inbreeding. Direct observation suggests that this male was the father of all infants born in the group since 1985. In 1991 individually identified samples of food wadges, hair, and feces were collected from most of the group members. These samples were analyzed using GT dinucleotide repeat polymorphisms to determine familial relationships. The genetic analysis revealed that the resident adult male was the probable father of only three out of the four infants analyzed. It is suspected that an adult male from a neighboring group was the father of the fourth infant, born in late 1986 or early 1987.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02382665
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