ISSN:
1573-9163
Keywords:
DNA methyltransferase
;
methylation single turnover
;
crosslinking of protein subunits
Source:
Springer Online Journal Archives 1860-2000
Topics:
Chemistry and Pharmacology
Notes:
Abstract The structural and catalytic properties of the phage T4 DNA-(adenine-N 6)-methyltransferase (EC 2.1.1.72) were studied at different enzyme–substrate concentration ratios by chemical crosslinking of the protein subunits and by measuring the presteady state kinetics of the reactions. Various structural states of the methyltransferase were correlated with its catalytic activity, and it was shown that the oligomeric forms of the enzyme are catalytically active but are characterized by the reaction parameters different from those of the monomer.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1023/A:1026618723150
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