ISSN:
1617-4623
Keywords:
Key wordsAspergillus
;
CCAAT
;
HapC
;
Hap complex
;
DNA binding
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract The Aspergillus nidulans hapC gene was expressed as a fusion protein with MalE or glutathione-S-transferase (GST) in Escherichia coli, and used for the purification of HapC and the preparation of anti-HapC antiserum. The CCAAT-binding factor AnCP/AnCF contains a component with an approximate molecular mass of 32 kDa that cross-reacts with the antibody. The MalE-HapC fusion protein was able to replace authentic HapC in AnCP when incubated under appropriate conditions. Furthermore, reconstitution experiments with recombinant HapC, yHAP2 and yHAP5 polypeptides showed that all three polypeptides were required for the assembly of a complex capable of binding to CCAAT-containing taaG2 promoter DNA. The relationship between AnCP/AnCF and the Saccharomyces cerevisiae HAP complex is discussed.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/s004380050664
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