ISSN:
1432-2242
Keywords:
Key words Restriction endonucleases
;
Chromatidaberrations
;
Intrachromosomal distribution
;
DNA
;
Hordeum vulgare
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract The intrachromosomal distribution patterns of chromatid aberrations induced by the restriction endonucleases HpaII, MspI and HaeIII in a multireconstructed barley karyotype were analysed. All of these endonucleases, which differ with respect to their DNA recognition sequences but produce only one type of DNA damage, showed nearly the same pattern of localized chromosome breakage. The most pronounced aberration hot spots proved to be the loci of transcriptionally active and condensed, inactive, rDNA. Possible mechanisms involved in the specific distribution of induced aberrations along the distinct chromosomal regions are discussed.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/s001220050496
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