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  • 1
    Electronic Resource
    Electronic Resource
    The properties of transketolase from photosynthetic tissue (1982)
    Springer
    Planta 155 (1982), S. 316-320 
    Details
    ISSN: 1432-2048
    Keywords: Chloroplast ; Cytosol ; Spinacia ; Thiamin pyrophosphate ; Transketolase ; Triticum (transketolase)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Transketolase (E.C. 2.2.1.1.) has been partially purified from wheat (Triticum aestivum, cv. Sappo) and spinach (Spinacia oleracea) leaves. The fully-active enzyme is a tetramer of relative molecular mass (Mr) of 150 kMr requiring thiamin pyrophosphate for maximal activity, and dissociating into a 74 kMr dimer in its absence or in dilute solution. The chloroplastic transketolase (over 75% of the cellular total) is magnesium-stimulated but the cytosolic form is magnesium-insensitive. Both chloroplastic and cytosolic transketolase showed similar broad specificities towards several ketose phosphate substrates including fructose 6-phosphate and sedoheptulose 7-phosphate. Wheat and spinach leaf transketolases are not light-activated and closely resemble the yeast enzyme in many of their properties.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00429458
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  • 2
    Electronic Resource
    Electronic Resource
    Acetyl coenzyme A biosynthesis in the chloroplast (1982)
    Springer
    Planta 156 (1982), S. 84-88 
    Details
    ISSN: 1432-2048
    Keywords: Acetyl CoA (biosynthesis) ; Chloroplast ; Coenzyme A, acetyl ; Cytosol ; Fatty acid ; Spinacia (acetyl CoA synthesis)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Acetyl coenzyme A (CoA) biosynthesis in spinach chloroplasts has been investigated by following the incorporation of bicarbonate and acetate into fatty acids under a variety of conditions. Both substrates were readily incorporated into fatty acids in a light-dependent manner by intact photosynthesising chloroplasts, but when the concentrations of these substrates were adjusted to those found in vivo, i.e. 200 μM acetate, 10 μM bicarbonate, then acetate was found to supply carbon atoms for fatty acids biosynthesis via acetyl CoA at forty times the rate of bicarbonate. It is proposed that extra-chloroplastic free acetate is the pricipal substrate for chloroplasts acetyl CoA biosynthesis in spinach.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00393446
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    Paper (German National Licenses)
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