ISSN:
0749-1581
Keywords:
Cyclic depsipeptide
;
Inverse COLOC
;
Inverse HMQC
;
Semi-selective excitation
;
NMR assignments in peptides
;
Didemnin
;
Chemistry
;
Analytical Chemistry and Spectroscopy
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Chemistry and Pharmacology
Notes:
Several one- and two-dimensional techniques have been used to assign the 1H and 13C NMR spectra of the cyclic heptadepsipeptide didemnin A in DMSO. The composition and sequence of the amino acid residues was known beforehand and could be confirmed by heteronuclear proton-carbon long-range couplings using the COLOC experiment and a recently proposed new inverse technique using Gaussian-shaped pulses. The aliphatic methyl resonances of the isostatin, hydroxyisovalerylpropionyl, N-methylleucine and leucine residues were assigned by means of the TOCSY and NOESY techniques. Aliphatic carbon assignment was performed by inverse correlation via heteronuclear double quantum coherence.
Additional Material:
5 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/mrc.1260260613
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