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  • 1
    Electronic Resource
    Electronic Resource
    Cyanellar Ferredoxin-NADP+-oxidoreductase of Cyanophora paradoxa is encoded by the nuclear genome and synthesized on cytoplasmatic 80S ribosomes (1990)
    Springer
    Current genetics 17 (1990), S. 265-267 
    Details
    ISSN: 1432-0983
    Keywords: Cyanophora paradoxa ; Ferredoxin-NADP+-oxidoreductase ; Protein-import ; Evolution
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Cyanophora paradoxa is an important model organism for the study of the transition from endocytobiontic cyanobacteria to factual eukaryotic cell organelles. The cyanelles of these organisms possess cyanobacterial, as well as plastidic, characteristics. Although the transfer of cyanellar proteins from cytosolic into cyanellar space has been shown, the process of translocation of a known protein across the peptidoglycan layer and the envelope membranes has not been characterized. In this study we demonstrate that a specific and obligate cyanelle protein —Ferredoxin-NADP+-oxidoreductase (FNR) — is coded on the nuclear genome, synthesized on 80S ribosomes and transported from the eukaryotic cell compartment into the cyanelles of Cyanophora paradoxa, an original intracellular host-guest relation. These results indicate a gene transfer from guest to host genome and support the view that, in spite of their cyanobacterial origin, cyanelles have been evolved to cell organelles comparable to plastids.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00312619
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Sequence analysis of pre-ferredoxin-NADP+-reductase cDNA from Cyanophora paradoxa specifying a precursor for a nucleus-encoded cyanelle polypeptide (1993)
    Springer
    Plant molecular biology 21 (1993), S. 1023-1033 
    Details
    ISSN: 1573-5028
    Keywords: cyanelles ; Cyanophora paradoxa ; peptidoglycan ; petH ; pre-ferredoxin-NADP+ reductase ; protein import
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A cDNA clone for pre-ferredoxin-NADP+ reductase (FNR) was obtained by screening a Cyanophora paradoxa expression library with antibodies specific for cyanelle FNR. The 1.4 kb transcript was derived from a single-copy gene. The precursor (41 kDa) and mature forms (34 kDa) of FNR were identified by western blotting of in vitro translation products and cyanelle extracts, respectively. The derived amino acid sequence of the mature form was corroborated by data from N-terminal protein sequencing and yielded identity scores from 58% to 62% upon comparison with cyanobacterial FNRs. Sequence conservation seemed to be even more pronounced in comparison with enzymes from higher plants, but using the neighbor joining method the C. paradoxa sequence was clearly positioned between the prokaryotic and eukaryotic sequences. The transit peptide of 65 or 66 amino acids appeared to be totally unrelated to those from spinach, pea and ice plant but showed overall characteristics of stroma-targeting peptides.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00023600
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  • 3
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    Brayton Power Conversion System Parametric Design Modelling for Nuclear Electric Propulsion (1993)
    In:  CASI
    Details
    Publication Date: 2019-06-28
    Description: The parametrically based closed Brayton cycle (CBC) computer design model was developed for inclusion into the NASA LeRC overall Nuclear Electric Propulsion (NEP) end-to-end systems model. The code is intended to provide greater depth to the NEP system modeling which is required to more accurately predict the impact of specific technology on system performance. The CBC model is parametrically based to allow for conducting detailed optimization studies and to provide for easy integration into an overall optimizer driver routine. The power conversion model includes the modeling of the turbines, alternators, compressors, ducting, and heat exchangers (hot-side heat exchanger and recuperator). The code predicts performance to significant detail. The system characteristics determined include estimates of mass, efficiency, and the characteristic dimensions of the major power conversion system components. These characteristics are parametrically modeled as a function of input parameters such as the aerodynamic configuration (axial or radial), turbine inlet temperature, cycle temperature ratio, power level, lifetime, materials, and redundancy.
    Keywords: SPACECRAFT PROPULSION AND POWER
    Type: NASA-CR-191135 , NAS 1.26:191135 , REPT-93RC-010143 , E-8345
    Format: application/pdf
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    NASA TECHNICAL REPORTS
  • 4
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    Engine Data Interpretation System (EDIS), phase 2 (1991)
    In:  CASI
    Details
    Publication Date: 2019-07-13
    Description: A prototype of an expert system was developed which applies qualitative constraint-based reasoning to the task of post-test analysis of data resulting from a rocket engine firing. Data anomalies are detected and corresponding faults are diagnosed. Engine behavior is reconstructed using measured data and knowledge about engine behavior. Knowledge about common faults guides but does not restrict the search for the best explanation in terms of hypothesized faults. The system contains domain knowledge about the behavior of common rocket engine components and was configured for use with the Space Shuttle Main Engine (SSME). A graphical user interface allows an expert user to intimately interact with the system during diagnosis. The system was applied to data taken during actual SSME tests where data anomalies were observed.
    Keywords: SPACECRAFT PROPULSION AND POWER
    Type: NASA-CR-184279 , NAS 1.26:184279 , UAH-ME-91-101
    Format: application/pdf
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    NASA TECHNICAL REPORTS
  • 5
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    Engine Data Interpretation System (EDIS) (1990)
    In:  CASI
    Details
    Publication Date: 2019-07-13
    Description: A prototype of an expert system was developed which applies qualitative or model-based reasoning to the task of post-test analysis and diagnosis of data resulting from a rocket engine firing. A combined component-based and process theory approach is adopted as the basis for system modeling. Such an approach provides a framework for explaining both normal and deviant system behavior in terms of individual component functionality. The diagnosis function is applied to digitized sensor time-histories generated during engine firings. The generic system is applicable to any liquid rocket engine but was adapted specifically in this work to the Space Shuttle Main Engine (SSME). The system is applied to idealized data resulting from turbomachinery malfunction in the SSME.
    Keywords: SPACECRAFT PROPULSION AND POWER
    Type: NASA-CR-184417 , NAS 1.26:184417 , UAH-RR-ME-90-101
    Format: application/pdf
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    NASA TECHNICAL REPORTS
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