ISSN:
0935-6304
Keywords:
Nonaqueous solvents
;
Capillary electrophoresis
;
Capillary electrochromatography
;
Current measurement
;
Chemistry
;
Analytical Chemistry and Spectroscopy
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Chemistry and Pharmacology
Notes:
The solvents acetonitrile, methanol, N, N-dimethylformamide, dimethyl sulfoxide, formamide, and deionized water were investigated for their ability to support current flow without added electrolyte. Using open tubular capillary electrophoresis, currents were measured to be in the nanoampere (10-9 A) range for all solvents but formamide (10-6 A). Comparisons with flow data showed no clear relationship between current and electroosmotic flow. Packed capillary columns (3-μm ODS) were used for separations using both pure solvent and hydrophobic dyes showed mild retention in pure ACN. A 16 polynuclear aromatic hydrocarbon (PAH) standard soluton was separated in 80/20 ACN/H2O with reduced plate heights (h) between 2.8 and 3.1 for retained species. A separation of nine androstenediones was achieved using a 70/30 MeOH/H2O mobile phase.
Additional Material:
6 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/jhrc.1240201003
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