ISSN:
1432-2048
Keywords:
Cucurbita
;
Cytokinin
;
Gene expression
;
Hydroxypyruvate reductase
;
Transcription
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract To understand the mechanisms by which the expression of a specific gene is modulated by cytokinin, the regulation of hydroxypyruvate reductase (HPR) transcript levels by N6-benzyladenine (BA) in etiolated pumpkin (Cucurbita pepo L. cv. Halloween) cotyledons was investigated. A pumpkin HPR cDNA was generated by reverse transcriptase-polymerase chain reaction and its nucleotide sequence was determined. An antisense HPR RNA was prepared for RNase protection analysis of HPR-mRNA expression patterns in the cotyledons of dark-grown pumpkin seedlings. Treatment of the cotyledons with BA was shown to modulate HPR mRNA levels in a dose- and time-dependent manner. Similarly, nuclear run-on studies showed that the rate of transcription was also enhanced by BA treatment of the cotyledons. These results suggest that the enhancement of HPR mRNA by cytokinin is, at least in part, at the level of transcription.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00197579
Permalink
