ISSN:
1617-4623
Keywords:
Crown gall tumors
;
Zein
;
Agrobacterium tumefaciens
;
Gene expression
;
Transgenic plants
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Summary Genes encoding maize seed storage proteins of 19 and 15 kd have been inserted at various sites in the T-DNA of the Ti plasmid pTiA6, Agrobacterium tumefaciens strains harboring these plasmids were used to incite tumors on sunflower stem sections. Some of the resulting tumors were found to contain mRNAs transcribed from the zein genes. The transcripts from both zein genes initiated and terminated at precisely the same sites in both maize endosperm and sunflower tumors. The steady state level of zein mRNAs in tumors varied from undetectable to approximately 1% of the level in maize endosperm. This variation between tumors in zein mRNA levels did not correlate with either zein gene copy number of the level of octopine synthase mRNA. However, in six tumor lines containing a zein gene inserted at the same site in TR, the level of zein mRNA was directly proportional to the level of a TR encoded mRNA.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00333265
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